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Object e.g. living cell, detection method for use in e.g. analysis of cell cultures, involves illuminating object, and imaging object by phase-shifting holographic interferometry for determination of complex-valued wave field of object
Object e.g. living cell, detection method for use in e.g. analysis of cell cultures, involves illuminating object, and imaging object by phase-shifting holographic interferometry for determination of complex-valued wave field of object
The method involves detecting a complex-valued image of an object, and determining a characteristic of the object by the analysis of the image. The object is illuminated for image detection in transmission. The object is imaged by a phase-shifting holographic interferometry for determination of the complex-valued wave field of the object. The complex-valued image is recorded by using a digital holography. The object wavefield of different focus levels are calculated by a numerical process with retention of a physical focus position. An independent claim is also included for a device for automatic object detection.
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