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BACTERIUM STRAIN LACTOCOCCUS LACTIS SUBCPECIES LACTIS B-8558 USEFUL IN DAIRY PRODUCT PREPARATION AND METHOD FOR PRODUCTION OF STARTER CULTURE OF STRAIN LACTOCOCCUS LACTIS SUBCPECIES LACTIS B-8558
BACTERIUM STRAIN LACTOCOCCUS LACTIS SUBCPECIES LACTIS B-8558 USEFUL IN DAIRY PRODUCT PREPARATION AND METHOD FOR PRODUCTION OF STARTER CULTURE OF STRAIN LACTOCOCCUS LACTIS SUBCPECIES LACTIS B-8558
FIELD: biotechnology, in particular dairy industry with high rheologic and quality characteristics.;SUBSTANCE: invention relates to new natural strain of lactic acid bacteria capable of exopolysaccharide synthesis in amount sufficient to improve rheologic product characteristics without introducing of food supplement and to increase functional properties of products producing with said strain. Starter culture of claimed strain is produced by fermentation under condition of semi-continuous deep culturing on broth containing milky whey, concentrated hydrolyzed milk, KH2PO4, Na2HPO4, MgSO4, sodium citrate, and distilled water. Fermentation is carried out at 22-32°C, constant pH 6.0-7.0 up to accumulation of living cell amount of 8.4-9.5 lg CFU/cm3. Biomass is separated, resuspended in protective medium, wherein as cryoprotector defatted milk with increased mass part of dry matter (up to 15 %) or defatted milk blended with gelatin in equal ratio is used; frozen and lyophilized. After drying biomass in bottles is sealed or pre-packed in sealed packing materials.;EFFECT: starter culture with maximal activity; products with improved quality and prolonged storage time.;2 cl, 2 dwg, 3 ex
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机译:技术领域:本发明涉及新的乳酸菌天然菌株,其能够胞外多糖合成,其量足以在不引入食品添加剂的情况下改善流变学产品特性并增加功能特性用所述菌株生产的产品。要求保护的菌株的起始培养是在含乳清乳清,浓缩水解乳,KH 2 Sub> PO 4 Sub>,Na 的肉汤中半连续深培养的条件下发酵而产生的2 Sub> HPO 4 Sub>,MgSO4 4 Sub>,柠檬酸钠和蒸馏水。在22-32℃,恒定pH 6.0-7.0下进行发酵,直至活细胞积累8.4-9.5 lg CFU / cm 3 Sup>。分离生物质,重悬于保护性介质中,其中冻干剂的脱脂奶用干物质的质量分数增加(至多15%)或与明胶等比例混合的脱脂奶用作冷冻保护剂。冷冻并冻干。干燥后,将瓶中的生物质密封或预先包装在密封的包装材料中。产品具有更高的质量和更长的存储时间。; 2 cl,2 dwg,3 ex
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