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Detection method for specific biomolecular interactions using FRET between metal nanoparticle and quantum dot

机译:利用FRET检测金属纳米粒子与量子点之间特定生物分子相互作用的方法

摘要

The present invention, a method for utilizing a pair of characteristics of the biomolecule to a pair of the energy receptor and the energy donor, specific binding indicating the FRET phenomenon, ① unknown determine whether specific binding between biomolecules ② a target molecule sample a method for detecting the presence of, and ③ a method for quantification of a target molecule present in the sample. ; According to the invention, which screen the biochemical substance in a quick and easy way without the labeling of the sample inhibit the specific binding between the pair of biomolecules with high speed and can be quantitatively analyzed and applied in new drug development, production in a number of cell can be applied or the like changes and quality inspection of the protein equivalent protein which is produced with the characteristic changes in accordance with analysis of the protein of the sugar used as a new drug candidate ; The metal nano-particles, quantum dots, fluorescent energy gongmyeongjeon this, glycoprotein, and high-speed screening
机译:本发明是一种利用生物分子对一对能量受体和能量供体的特征的方法,其特异性结合表明FRET现象,①未知确定生物分子之间是否特异性结合②目标分子样品的方法③检测样品中存在的靶分子的方法。 ;根据本发明,以快速,容易的方式筛选生化物质而无需标记样品,从而抑制了这对生物分子之间的特异性结合,并且可以被定量地分析并应用于新药开发,生产中。根据对用作新药候选糖的蛋白质的分析,可以对细胞进行改变或类似的改变,并检验具有特征改变的蛋白质等效蛋白的质量;金属纳米粒子,量子点,荧光能量公明,糖蛋白和高速筛选

著录项

  • 公开/公告号KR100704011B1

    专利类型

  • 公开/公告日2007-04-04

    原文格式PDF

  • 申请/专利权人

    申请/专利号KR20060012213

  • 申请日2006-02-08

  • 分类号G01N33/52;B82B3/00;

  • 国家 KR

  • 入库时间 2022-08-21 20:32:28

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