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IN VITRO EVALUATION OF MICRO-ORGANISMS AND THEIR ANTIMICROBIAL AGENT SUSCEPTIBILITIES

机译:微生物的体外评估及其抗菌剂的敏感性

摘要

A method of identifying a microorganism and determining the susceptibility of the microorganism to an antimicrobial agent by inserting a sample of the microorganism in a primary culture container and allowing the microorganism to multiply. The primary culture is then divided into a plurality of secondary cultures and the metabolic volatile or semi-volatile compounds (VCs) in the headspace above the cultures are analysed by SIFT-MS to ascertain whether micro-organisms exist in the culture and determine the type of micro-organism. The secondary cultures are then divided into a number of tertiary cultures and an antimicrobial agent is introduced whereupon the VCs in the headspace above the tertiary cultures are analysed by SIFT-MS to determine the susceptibility of the microorganism to the antimicrobial agent at various concentrations of the antimicrobial agent.
机译:一种通过将微生物样品插入初级培养容器中并使微生物繁殖来鉴定微生物并确定微生物对抗菌剂的敏感性的方法。然后将原代培养物分为多个二次培养物,并通过SIFT-MS分析培养物上方顶空的代谢性挥发性或半挥发性化合物(VC),以确定培养物中是否存在微生物并确定其类型微生物。然后将继代培养物分为多种第三代培养物,并引入抗菌剂,然后通过SIFT-MS分析第三代培养物上方空间中的VC,以测定在各种浓度的微生物对抗菌剂的敏感性。抗菌剂。

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