首页> 外国专利> USE OF REAL TIME PCR FOR DETECTION OF ALLELIC EXPRESSION

USE OF REAL TIME PCR FOR DETECTION OF ALLELIC EXPRESSION

机译：使用实时荧光定量PCR检测等位基因表达

页面导航

摘要
著录项
相似文献

摘要

The present invention relates, e.g., to a method for quantitating the relative amounts of a plurality of different RNA transcripts expressed from a gene of interest (such as expression products of two alleles of a gene of interest), e.g. in a subject or cell, comprising performing real time quantitative PCR, in a single container, of cDNAs of the transcripts, in the presence of probes specific for each of the different transcripts, wherein the probes for each transcript are labeled with distinguishable fluorophores. The amount of expression of each of the plurality of transcripts may be quantitated by comparison to a standard curve obtained by real time quantitative PCR of mixtures of different ratios of isolated DNAs representing each of the plurality of transcripts. Methods for validating the effectiveness and specificity of allele-specific siRNAs, and methods of diagnosis, are also described, as are kits for performing methods of the invention.

机译：本发明涉及例如定量从目的基因（例如目的基因的两个等位基因的表达产物）表达的多个不同RNA转录物的相对量的方法。在受试者或细胞中，包括在存在针对每个不同转录物的特异性探针的情况下，在单个容器中对转录物的cDNA进行实时定量PCR，其中每个转录物的探针用可区分的荧光团标记。多个转录物的每一个的表达量可以通过与通过实时定量PCR对代表多个转录物的每一个的不同比例的分离DNA的混合物进行实时定量PCR获得的标准曲线进行比较来定量。还描述了用于验证等位基因特异性siRNA的有效性和特异性的方法以及诊断方法，以及用于进行本发明方法的试剂盒。

著录项

公开/公告号WO2007035697A1

专利类型
公开/公告日2007-03-29

原文格式PDF
申请/专利权人 THE GOVERNMENT OF THE UNITED STATES OF AMERICA AS REPRESENTED BY THE SECRETARY DEPARTMENT OF HEALTH AND HUMAN SERVICES;HUIZING MARJAN;KLOOTWIJK ENRIKO;SAVELKOUL PAUL J.M.;CICCONE CARLA;GAHL WILLIAM;
展开▼

申请/专利号WO2006US36411
发明设计人 KLOOTWIJK ENRIKO;SAVELKOUL PAUL J.M.;HUIZING MARJAN;CICCONE CARLA;GAHL WILLIAM;
展开▼

申请日2006-09-20
分类号C12Q1/68;
国家 WO
入库时间 2022-08-21 20:50:36

相似文献

专利
外文文献
中文文献