首页> 外国专利> METHODS OF INDUCING THE EXPRESSION OF BONE MORPHOGENETIC PROTEINS (BMPs) AND TRANSFORMING GROWTH FACTOR-beta-PROTEINS (TGF-betas) IN CELLS

METHODS OF INDUCING THE EXPRESSION OF BONE MORPHOGENETIC PROTEINS (BMPs) AND TRANSFORMING GROWTH FACTOR-beta-PROTEINS (TGF-betas) IN CELLS

机译：诱导细胞中骨形态发生蛋白（BMPs）表达和转化生长因子-β-蛋白（TGF-β）的方法

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摘要

A method of inducing the expression of one or more bone morphogenetic proteins and/or transforming growth factor-beta proteins in a cell is described. The method includes transfecting a cell with an isolated nucleic acid comprising a nucleotide sequence encoding a LIM mineralization protein operably linked to a promoter. The one or more bone morphogenetic proteins can be BMP-2, BMP-4, BMP-6, BMP-7 or combinations thereof. The transforming growth factor-beta protein can be transforming growth factor-beta1 protein (TGF-beta1). Transfection may be accomplished ex vivo or in vivo by direct injection of virus or naked DNA, or by a nonviral vector such as a plasmid. The method can be used to induce bone formation in osseous cells or to stimulate proteoglycan and/or collagen production in cells capable of producing proteoglycyan and/or collagen (e.g., intervertebral disc cells).

机译：描述了一种在细胞中诱导一种或多种骨形态发生蛋白表达和/或转化生长因子-β蛋白的方法。该方法包括用分离的核酸转染细胞，所述分离的核酸包含编码与启动子可操作连接的LIM矿化蛋白的核苷酸序列。一种或多种骨形态发生蛋白可以是BMP-2，BMP-4，BMP-6，BMP-7或其组合。转化生长因子β蛋白可以是转化生长因子β1蛋白（TGF-β1）。转染可通过直接注射病毒或裸露的DNA，或通过非病毒载体例如质粒来离体或体内完成。该方法可用于在骨细胞中诱导骨形成或在能够产生蛋白蓝和/或胶原的细胞（例如椎间盘细胞）中刺激蛋白聚糖和/或胶原的产生。

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公开/公告号EP1629106A4

专利类型
公开/公告日2006-12-06

原文格式PDF
申请/专利权人 MEDTRONIC SOFAMOR DANEK;
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申请/专利号EP20040749373
发明设计人 MCKAY WILLIAM;BODEN SCOTT;YOON SANGWOOK;
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申请日2004-03-07
分类号C12P21/06;A01N43/04;A01N63;A61F2/28;A61F2/30;A61F2/44;A61K35/12;A61K35/13;A61K38/17;A61K48;A61L27/38;C07K14/47;C07K14/475;C07K14/51;C07K14/78;C07K16/18;C12N;C12N5;C12N5/08;C12N15/63;C12N15/70;C12N15/861;C12N15/867;C12P21/02;
国家 EP
入库时间 2022-08-21 20:51:03

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