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ARRAY COMPARATIVE GENOMIC HYBRIDISATION METHODS USING LINEAR AMPLIFICATION
ARRAY COMPARATIVE GENOMIC HYBRIDISATION METHODS USING LINEAR AMPLIFICATION
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机译:线性扩增的阵列比较基因组杂交方法
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摘要
In a CGH array method, amplification of genomic DNA proceeds linearly rather than exponentially. In vitro transcription using T7 RNA polymerase is particularly suitable. Using a linear amplification method avoids the bias associated with prior art methods. Amplification preferably follows restriction fragmentation. Preferred restriction enzymes produce degenerate cohesive ends, which allows complexity reduction of the products by the use of specific linkers, and in particular linkers that include a T7 RNA polymerase promoter.
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