首页> 外国专利> RECOMBINANT EXPRESSION OF RUBELLA E1 ENVELOPE PROTEIN VARIANTS AS CHAPERONE FUSION-PROTEINS, THEIR REFOLDING INTO A SOLUBLE, NATIVE-LIKEAND IMMUNOREACTIVE ANTIGENIC CONFORMATION AND THEIR USE IN THE DETECTION OFANTI-RUBELLA ANTIBODIES

RECOMBINANT EXPRESSION OF RUBELLA E1 ENVELOPE PROTEIN VARIANTS AS CHAPERONE FUSION-PROTEINS, THEIR REFOLDING INTO A SOLUBLE, NATIVE-LIKEAND IMMUNOREACTIVE ANTIGENIC CONFORMATION AND THEIR USE IN THE DETECTION OFANTI-RUBELLA ANTIBODIES

机译：RUBELLA E1信封蛋白变体作为伴侣融合蛋白的重组表达，将其重溶为可溶的，天然的免疫原性抗原构象，并用于检测抗-RUBELLA抗体

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摘要

The invention relates to a soluble Rubella E1 antigen and variants lacking a t the C--terminal end at least the transmembran region and the anchor segment as well as at least the amino acids 143 to 164 and containing at least the region spanning the disulfid-bridges Cys 349 - Cys 352 and Cys 368 - 401 whereas the N-terminus of this region contains additionally at least 15 amino acids and/or the C- terminus of this region contains additionally at least 8 amino acids of the adjacent Rubella E1 antigen sequence. The region spanning the disulfid-bridges Cys 349 - Cys 352 and Cys 368 - 40 1 contains at the N-terminus of this region additionally at least 25, 30, 34 amino acids and/or at the C-terminus of this region additionally at least 10, 11, 15, 25 , 35 amino acids of the adjacent Rubella E1 antigen sequence. Furthermore, the invention relates to a recombinant DNA molecule encoding a Rubella E1 antigen and variants, methods for the detection, determination an d quantification of anti-Rubella antibodies, and a diagnostic test and reagent kit for the detection of anti-Rubella antibodies.

机译：本发明涉及可溶性风疹E1抗原和变异体，其在C-末端缺乏至少跨膜区和锚链节以及至少氨基酸143至164，并且至少包含跨越二硫键的区域Cys 349-Cys 352和Cys 368-401，而该区域的N末端还包含至少15个氨基酸，和/或此区域的C末端还包含相邻风疹E1抗原序列的至少8个氨基酸。跨越二硫桥Cys 349-Cys 352和Cys 368-40 1的区域在该区域的N末端还包含至少25、30、34个氨基酸和/或在该区域的C末端另外包含。相邻风疹E1抗原序列的至少10、11、15、25、35个氨基酸。此外，本发明涉及编码风疹E1抗原和变体的重组DNA分子，检测，确定抗风疹抗体的量和定量的方法以及用于检测抗风疹抗体的诊断测试和试剂盒。

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公开/公告号CA2562738A1

专利类型
公开/公告日2007-04-26

原文格式PDF
申请/专利权人 F. HOFFMANN-LA ROCHE AG;
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申请/专利号CA20062562738
发明设计人 FAATZ ELKE;SCHAARSCHMIDT PETER;BOLLHAGEN RALF;ENGEL ALFRED;SCHOLZ CHRISTIAN;ZARNT TORALF;UPMEIER BARBARA;
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申请日2006-10-23
分类号C12N15/62;A61K39/20;C07K14/19;C07K19;C12N15/31;C12N15/61;C12N15/63;C12P21/02;G01N33/53;G01N33/569;
国家 CA
入库时间 2022-08-21 20:53:54

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