首页> 外国专利> METHOD OF CONSTRUCTING AMINO ACID PRODUCING BACTERIA, AND METHOD OF PREPARING AMINO ACIDS BY FERMENTATION WITH THE CONSTRUCTED AMINO ACID PRODUCING BACTERIA

METHOD OF CONSTRUCTING AMINO ACID PRODUCING BACTERIA, AND METHOD OF PREPARING AMINO ACIDS BY FERMENTATION WITH THE CONSTRUCTED AMINO ACID PRODUCING BACTERIA

机译：构建氨基酸生产细菌的方法，以及通过发酵构建氨基酸生产细菌制备氨基酸的方法

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摘要

A METHOD OF PRODUCING CORYNEFORM BACTERIA HAVING AN IMPOVED AMINO ACID-OR NUCLEIC ACID-PRODUCTIVITY COMPRISES THE STEPS OF CAUSING A MUTATION IN A PROMOTER SEQUENCE OF AMINO ACID-OR NUCLEIC ACID-BIOSYNTHESIZING GENES ON A CHROMOSOME OF A CORYNEFORM BACTERIUM TO MAKE IT CLOSE TO A CONSENSUS SEQUENCE OR INTRODUCING A CHANGE IN A PROMOTER SEQUENCE OF AMINO ACID-BIOSYNTHESIZING GENES ON A CHROMOSOME OF A CORYNEFORM BACTERIUM BY THE GENE RECOMBINATION TO MAKE IT CLOSE TO A CONSENSUS SEQUENCE, TO OBTAIN MUTANTS OF THE CORYNEFORM AMINO ACID-OR NUCLEIC ACID-PRODUCING MICROORGANISM, CULTURING THE MUTANTS AND SELECT A MUTANT CAPABLE OF PRODUCING THE INTENDED AMINO ACID OR NUCLEIC ACID IN A LARGE AMOUNT. THIS METHOD CAN CONSTRUCT A MUTANT CAPABLE OF SUITABLY ENRICHING OR CONTROLING THE EXPRESSION OF AN INTENDED GENE WITHOUT USING A PLASMID AND ALSO CAPABLE OF PRODUCING AMINO ACIDS INA HIGH YIELD, BY THE RECOMBINATION OR MUTATION.

机译：一种生产具有提高的氨基酸或核酸生产率的葫芦形细菌的方法，该方法包括在密接的玉米粉状细菌染色体上的氨基酸序列中引起氨基酸或核酸生物合成基因突变的步骤。通过基因重组，在胭脂状细菌的染色体上通过氨基酸重组在氨基酸序列上一致的序列或引入促进氨基酸酸合成的基因的启动子序列的改变，从而使该核苷酸序列的氨基酸突变成乙酰丙酮酸产生微生物，培养突变体并选择能够以大量生产预期的氨基酸或核酸的突变体。该方法可以构建能够适当富集或控制目的基因表达的突变体，而无需使用质粒，并且还可以通过重组或诱变在高产氨基酸的情况下生产氨基酸。

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公开/公告号MY130756A

专利类型
公开/公告日2007-07-31

原文格式PDF
申请/专利权人 AJINOMOTO CO. INC.;
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申请/专利号MY1999PI04141
发明设计人 EIICHIRO KIMURA;OSAMU KURAHASHI;YOKO ASAKURA;JUN NAKAMURA;KAZUHIKO MATSUI;TSUYOSHI OHSUMI;TSUYOSHI NAKAMATSU;SOHEI KANNO;MIKIKO SUGA;HISAO ITO;
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申请日1999-09-24
分类号C12N1/21;C12N9/02;C12N9/10;C12N15/52;C12N15/67;C12P13/04;C12P13/14;C12P19/38;
国家 MY
入库时间 2022-08-21 20:55:58

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