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Protein-protein interactions and methods for identifying interacting proteins and the amino acid sequence at the site of interaction
Protein-protein interactions and methods for identifying interacting proteins and the amino acid sequence at the site of interaction
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机译:蛋白质间相互作用以及鉴定相互作用蛋白和相互作用位点氨基酸序列的方法
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摘要
The invention relates to protein-protein interactions and methods for identifying interacting proteins and the amino acid sequence at the site of interaction. Using overlapping hexapeptides that encode for the entire amino acid sequences of the linker domains of human P-glycoprotein gene 1 and 3 (HP-gp1 and HP-gp3), a direct and specific binding between HP-gp1 and 3 linker domains and intracellular proteins was demonstrated. The method of the present invention was further validated with Annexin. The present invention thus demonstrates a novel concept whereby the interactions between two proteins are mediated by strings of few amino acids with high and repulsive binding energies, enabling the identification of high affinity binding sites between any interacting proteins.
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