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Proteomic screening for redox state dependent protein—protein interactions
Proteomic screening for redox state dependent protein—protein interactions
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机译:蛋白质组学筛选氧化还原状态依赖性蛋白-蛋白相互作用
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摘要
This invention provides a modified yeast two-hybrid system in order to identify NO-dependent protein-protein interactions. Bait proteins implicated in apoptotic signaling pathways were used to identify NO-dependent interactions. The physiological relevance of these interactions is demonstrated by their occurrence and dependence on endogenous NO in mammalian cells, and by the functional interrelatedness of bait and prey.
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