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Amperometric sensor for uric acid and method for the same

机译:用于尿酸的安培传感器及其方法

摘要

An amperometric sensor for uric acid and a manufacturing method thereof are disclosed, in which polyacrylamide is used to fix catalase, uricase and ferrocenecarboxylic acid on a working electrode. In determining concentration of uric acid, hydrogen peroxide is produced when enzyme and uric acid react with each other and then a reduction current generated from enzyme on the electrode with an external voltage 200 mV applied is detected. In determining concentration of uric acid, a concentration range of 2.5-20 mg/dl is achieved and sensibility of the sensor in a linear portion is 5.17 uAcm−2(mg/dl)−1. In addition, reaction time required for the reaction between enzyme and uric acid is 5.17 uAcm−2(mg/dl)−1.
机译:公开了一种用于尿酸的安培传感器及其制造方法,其中使用聚丙烯酰胺将过氧化氢酶,尿酸酶和二茂铁羧酸固定在工作电极上。在确定尿酸的浓度时,当酶和尿酸相互反应时会产生过氧化氢,然后在施加200 mV外部电压的情况下检测到酶在电极上产生的还原电流。在测定尿酸浓度时,浓度范围为2.5-20 mg / dl,线性部分的传感器灵敏度为5.17 uAcm -2 (mg / dl) -1 。另外,酶与尿酸反应所需的反应时间为5.17uAcm -2 (mg / dl) -1

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