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Brief description of the dissociation of the molecule of extracellular hemoglobin of arenicola marina, chains constituting the proteinic molecule and nucleotide sequences coding for the said protein chains

机译:滨藜的胞外血红蛋白分子的解离,构成蛋白质分子的链和编码所述蛋白质链的核苷酸序列的简要说明

摘要

Dissociating molecules of the extracellular hemoglobin (I) of Annelids, particularly Arenicola marina, to produce the 10 protein chains (II) that make up (I), comprises treating (I) with at least one dissociating agent (III). (III) is particularly a mixture of dithiothreitol; tris(2-carboxyethyl)phosphine or 2-mercaptoethanol with a dissociation buffer. Independent claims are also included for: (1) preparing a pair of primers (P) from (II); (2) pair of (P) produced by method (1); (3) preparing nucleic acid sequences (NA) that encode (I), using the primers of (1); (4) proteins encoded by NA; and (5) NA produced by method (3) or encoding the proteins of (4).
机译:解离Annilids的细胞外血红蛋白分子(I),特别是滨藜(Arenicola marina),以产生组成(I)的10条蛋白质链(II),包括用至少一种解离剂(III)处理(I)。 (III)特别是二硫苏糖醇的混合物;三(2-羧乙基)膦或2-巯基乙醇,带有解离缓冲液。还包括以下方面的独立权利要求:(1)从(II)制备一对引物(P); (2)用方法(1)制得的(P)对; (3)用(1)的引物制备编码(I)的核酸序列(NA)。 (4)NA编码的蛋白质; (5)通过方法(3)产生或编码(4)的蛋白质的NA。

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