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Brief description of the dissociation of the molecule of extracellular hemoglobin of arenicola marina, chains constituting the proteinic molecule and nucleotide sequences coding for the said protein chains
Brief description of the dissociation of the molecule of extracellular hemoglobin of arenicola marina, chains constituting the proteinic molecule and nucleotide sequences coding for the said protein chains
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机译:滨藜的胞外血红蛋白分子的解离,构成蛋白质分子的链和编码所述蛋白质链的核苷酸序列的简要说明
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摘要
Dissociating molecules of the extracellular hemoglobin (I) of Annelids, particularly Arenicola marina, to produce the 10 protein chains (II) that make up (I), comprises treating (I) with at least one dissociating agent (III). (III) is particularly a mixture of dithiothreitol; tris(2-carboxyethyl)phosphine or 2-mercaptoethanol with a dissociation buffer. Independent claims are also included for: (1) preparing a pair of primers (P) from (II); (2) pair of (P) produced by method (1); (3) preparing nucleic acid sequences (NA) that encode (I), using the primers of (1); (4) proteins encoded by NA; and (5) NA produced by method (3) or encoding the proteins of (4).
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