首页> 外国专利> Cytochrome p450 monooxygenase and nadph - cytochrome p450 - oxidoreductase genes and proteins with respect to the omega - hydroxylase - complex in candida tropicalis and associated method

Cytochrome p450 monooxygenase and nadph - cytochrome p450 - oxidoreductase genes and proteins with respect to the omega - hydroxylase - complex in candida tropicalis and associated method

机译：热带念珠菌中ω-羟化酶复合物的细胞色素p450单加氧酶和nadph-细胞色素p450氧化还原酶基因和蛋白及其相关方法。

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摘要

Novel genes have been isolated which encode cytochrome P450 and NADPH reductase enzyme of the φ-hydroxylase complex of C. tropicalis 20336. Vectors including these genes, transfected host cells and transformed host cells are provided. Methods of producing of cytochrome P450 and NADPH reductase enzymes are also provided which involve transforming a host cell with a gene encoding these enzymes and culturing the cells. Methods of increasing the production of a dicarboxylic acid and methods of increasing production of the aforementioned enzymes are also provided which involve increasing in the host cell the number of genes encoding these enzymes. A method for discriminating members of a gene family by quantifying the expression of genes is also provided.

机译：已分离出新的基因，它们编码热带假丝酵母20336的φ-羟化酶复合物的细胞色素P450和NADPH还原酶。提供了包括这些基因的载体，转染的宿主细胞和转化的宿主细胞。还提供了产生细胞色素P450和NADPH还原酶的方法，该方法包括用编码这些酶的基因转化宿主细胞并培养该细胞。还提供了增加二羧酸产量的方法和增加上述酶产量的方法，这些方法包括增加宿主细胞中编码这些酶的基因的数量。还提供了一种通过定量基因表达来区分基因家族成员的方法。

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公开/公告号DE69924873T2

专利类型
公开/公告日2006-03-02

原文格式PDF
申请/专利权人 COGNIS CORP. GULPH MILLS PA. US;
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申请/专利号DE1999624873T
发明设计人 ESHOO MARK BERKELEY US;MADDURI KRISHNA M. INDIANAPOLIS US;CRAFT L. DAVID FORT THOMAS US;WILSON RON C. LOVELAND US;EIRICH DUDLEY L. CINCINNATI US;CORNETT CATHY A. CRESCENT SPRINGS US;LOPER C. JOHN CINCINNATI US;BRENNER A. ALFRED SANTA ROSA US;GLEESON MARTIN SAN DIEGO US;TANG MARIA FAIRFIELD US;
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申请日1999-09-10
分类号C12N15/53;C12N9/02;C12N15/81;C12N5/10;C12Q1/68;C12P7/44;C12R1/74;
国家 DE
入库时间 2022-08-21 21:17:50

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