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Electrophoresis method, e.g. for analysis and preparation separation of ions, peptides, involves providing boundaries defining separation sub-spaces by adjacent flow of anodic and cathodic stabilization media through respective inlets
Electrophoresis method, e.g. for analysis and preparation separation of ions, peptides, involves providing boundaries defining separation sub-spaces by adjacent flow of anodic and cathodic stabilization media through respective inlets
The separation medium and samples are introduced into a separation chamber (100) through separation medium inlets (126a-126c), and sample inlets (140a-140c) respectively. An electric field is generated through anode (120) and cathode (122) in chamber, and anodic stabilization medium and cathodic stabilization medium are introduced into chamber through respective inlets (128a-128c, 130a-130c). The boundaries defining separation sub-spaces (136a-136c) are provided by adjacent flow of anodic and cathodic stabilization media through the respective inlets. The anodic stabilization medium comprises a monoprotic acid with anion having electrophoretic mobility less than or equal to 40x10 -9 m 2/V/sec. The cathodic stabilization medium contains a monobasic base with cation having electrophoretic mobility of less than or equal to 40x10 19 m 2/V/sec. The anodic stabilization medium has a pH about 0.5 to about 3 pH units less than the pH of the lowest-pH constituent of the separation medium, and the cathodic stabilization medium has a pH about 0.5 to about 3 pH units higher than the pH of the highest-pH constituent of the separation medium.
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机译：分离介质和样品分别通过分离介质入口（126a-126c）和样品入口（140a-140c）引入分离室（100）。通过腔室中的阳极（120）和阴极（122）产生电场，并且通过各自的入口（128a-128c，130a-130c）将阳极稳定介质和阴极稳定介质引入腔室。限定分离子空间（136a-136c）的边界由穿过相应入口的阳极和阴极稳定介质的相邻流动提供。阳极稳定介质包含单质子酸，其阴离子的电泳迁移率小于或等于40x10 -9> m 2> / V / sec。阴极稳定介质包含具有阳离子迁移率小于或等于40 x 10 19> m 2> / V / sec的阳离子的一元碱。阳极稳定介质的pH值比分离介质中最低pH成分的pH值小约0.5至约3个pH单位，阴极稳定介质的pH值比分离介质中最低pH值的pH值高约0.5至约3个pH单位。分离介质的最高pH成分。
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