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首页> 外国专利> RECOMBINANT PLASMID DNA ENCODING SYNTHESIS OF DELTA-ENDOTOXIN Cry IIIA AND STRAIN BACILLUS THURINGIENSIS SUBSPECIES KURSTAKI PREPARED ON RECOMBINANT PLASMID DNA BASE

RECOMBINANT PLASMID DNA ENCODING SYNTHESIS OF DELTA-ENDOTOXIN Cry IIIA AND STRAIN BACILLUS THURINGIENSIS SUBSPECIES KURSTAKI PREPARED ON RECOMBINANT PLASMID DNA BASE

机译：重组质粒DNA编码的δ-内毒素Cry IIIA和苏云金芽孢杆菌苏云金杆菌亚种的合成

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FIELD: microbiological industry, biotechnology.;SUBSTANCE: invention relates to production of bacterial preparations. Method involves construction of recombinant plasmid DNA comprising gene determining the resistance to erythromycin (ErmC), sequence of DNA fragment of B. thuringiensis subsp. tenebrionis encoding synthesis of δ-endotoxin cry IIIA, sequence of DNA fragment of chromosome B. thuringiensis subsp. kurstaki of size 448 nucleotide pairs, sequence of DNA fragment of chromosome B. thuringiensis subsp. kurstaki of size 501 nucleotide pairs, and sites for cleavage with restriction site-specific endonucleases Eco RI, Pst I, Bam HI, Hind III and Kpn I. The strain B. thuringiensis subsp. kurstaki comprising the indicated recombinant plasmid DNA is prepared. Invention provides preparing the strain B. thuringiensis possessing the enhanced insecticide activity with respect to representatives of Lepidoptera, Coleoptera and Homoptera orders showing the damage effects on agriculture cultures and crops.;EFFECT: valuable properties of plasmid DNA.;3 cl, 5 dwg, 6 tbl, 4 ex
机译：技术领域：微生物工业，生物技术;发明领域：本发明涉及细菌制剂的生产。该方法涉及构建重组质粒DNA，该重组质粒DNA包含确定对红霉素（ErmC）的抗性，苏云金芽孢杆菌亚种DNA片段序列的基因。编码内生毒素cry IIIA的合成的tenebrionis，苏云金芽孢杆菌B亚种的DNA片段序列。 kurstaki，大小为448个核苷酸对，苏云金芽孢杆菌B亚种的DNA片段序列。苏云金芽胞杆菌的大小为501个核苷酸对，并具有限制性位点特异性内切核酸酶Eco RI，Pst I，Bam HI，Hind III和Kpn I的切割位点。制备包含所示重组质粒DNA的库尔斯塔基。本发明提供了针对鳞翅目，鞘翅目和同翅目代表的具有增强的杀虫活性的苏云金芽孢杆菌菌株的制备，其显示出对农业培养物和农作物的破坏作用。 6汤匙，4前

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