METHOD OF INCREASING viability of eggs, larvae and juvenile fish

• 1. 一种黑水虻幼虫替代鱼粉的巴沙鱼幼鱼饲料及其制备方法 [P] . 中国专利： CN111387373A . 2020-07-10
• 2. 一种提高大菱鲆受精卵孵化率和仔稚幼鱼生长的光环境调控方法 [P] . 中国专利： CN108541633B . 2020.10.30
• 3. METHOD FOR INCREASING VIABILITY OF EGGS, LARVAE AND JUVENILE FISH [P] . 俄罗斯专利： RU2613971C1 . 2017-03-22

  机译： 提高鸡蛋，幼鱼和幼鱼活度的方法

• 4. METHOD FOR INCREASING VIABILITY OF CAVIAR, LARVAE, YOUNG FISHES AND ACCELERATION OF THEIR GROWTH [P] . 俄罗斯专利： RU2223643C2 . 2004-02-20

  机译： 提高鱼子酱，幼体，幼鱼的活力并促进其生长的方法

• 5. ADHESION/GROWTH PROMOTION MATERIAL FOR SHELLFISH EGGS AND SHELLFISH LARVAE AND SHELLFISH COLLECTION METHOD AND CULTIVATION METHOD USING SAME [P] . 世界知识产权组织专利： WO2015105200A1 . 2015-07-16

  机译： 贝壳类蛋和贝壳类幼虫的附着/生长促进材料以及贝壳类的收集方法和栽培方法

• 1. Spatiotemporal distribution of fish eggs and larvae in the Huanghe (Yellow) River estuary, China in 2005-2016 [J] . SONG Yingfei, ZHANG Longjun, LUO Xianxiang 海洋湖沼学报（英文） . 2019,第005期
• 2. Species Composition and Protection of Fish Eggs and Larvae in Spawning Ground of Liaodong Bay [J] . Yu Xuguang, Dong Jing, Wang Bin, 动物与饲料科学：英文版 . 2018,第003期
• 3. Experimental Analysis on Allometric Growth in Spualiobarbus curriculus Larvae and Juveniles in Zhaoqing Reach of the Pearl River [J] . Chen Fangcan, Li Xinhui, Li Jie, 动物与饲料科学：英文版 . 2018,第001期
• 4. Effect of various sources of carotenoids on survival and growth of goldfish (Carassius auratus) larvae and juveniles [C] . P. Rema, L. Gouveia, J. Empis ESMB meeting . 2002
• 5. Effects of zooplankton community structure and nutritional value on the eggs and larvae of two estuarine fish species. [D] . Volson, Barry. 2012
• 6. Morphological Development of Eggs Larvae and Juveniles of the FarEastern Catfish Silurus asotus in Korea (Pisces:Siluridae) [O] . Seong Jun Mun, Hu Sun Yim, Kyeong Ho Han, 2017
• 7. Eggs, larvae and juveniles of Indian Scombroid fishes [O] . Jones S, Kumaran M 1964
• 8. Fish Eggs and Larvae of the Chesapeake Bay. I. Retention by Nets of Differing Mesh Sizes. II. Observations on the Problem of Identifying Field Mortalities of Striped Bass (Morone saxatilis) Larvae from Preserved Samples [R] . Otto, R. G. , Kaufman, L. S. , Boggs, J. A. 1980

• 1. BACKGROUND： Vibrio vulnifi cus inside the body could activate the NF-!B signaling pathwayand initiate the inflammatory cascade. The lung is one of the earliest organs affected by sepsisassociated with acute lung injury. High mobility group protein B1 （HMGB1） is an important late-actingpro-infl ammatory cytokine involving in the pathophysiology of sepsis. It is also involved in the injuryprocess in the lung, liver and intestine. There has been no report on the involvement of HMGB1 inVibrio vulnifi cus sepsis-induced lung injury.METHODS： Sixty rats were randomly divided into a normal control group （group A, n=10） anda Vibrio vulnificus sepsis group （group B, n=50）. Sepsis was induced in the rats by subcutaneousinjection of Vibrio vulnificus （concentration 6×108 cfu/mL, volume 0.1 mL/100g）） into the left lowerlimbs. The rats in group B were sacrifi ced separately 1, 6, 12, 24, and 48 hours after the infection.Their lungs were stored as specimens, lung water content was measured, and lung pathology wasobserved under a light microscope. The expressions of the HMGB1 gene and protein in the lungswere detected by RT-PCR and Western blot. Data were analyzed with one-way analysis of variance（ANOVA） and the LSD method for pair-wise comparison between the two groups. P〈0.05 wasconsidered statistically signifi cant.RESULTS： Compared to group A （0.652±0.177）, HMGB1 mRNA expression in the lungs ofgroup B was signifi cantly higher at 0 hour （1.161±0.358, P=0.013）, 24 hours （1.679±0.235, P=0.000）,and 48 hours （1.258±0.274, P=0.004） （P〈0.05）, and peaked at 24 hours. Compared to group A（0.594±0.190）, HMGB1 protein expression at 6 hours （1.408±0.567, P=0.026） after infection wassignificantly increased （P〈0. 05）, and peaked at 24 hours （2.415±1.064, P=0.000） after infection.Compared to group A （0.699±0.054）, lung water content was significantly increased at 6 hours（0.759±0.030, P=0.001）,12 hours （0.767±0.023, P=0.000）, 24 hours （0.771±0.043, P=0.000） and 48hours （0.789±0.137, P=0.000） after infection （P〈0.05）. Compared to group A, pathological changesat 12 hours in group B indicate marked pulmonary vascular congestion, interstitial edema andinfl ammatory infi ltration. Alveolar cavity collapse and boundaries of the alveolar septum could not beclearly identifi ed.CONCLUSION： Vibrio vulnifi cus sepsis can lead to injury in rat lungs, and increased HMGB1expression in lung tissue may be one of the mechanisms for injury from Vibrio vulnifi cus sepsis. [J] . Qiao-meng Qiu ,Zhong-wang Li ,Lu-ming Tang . 世界急诊医学杂志(英文) . 2011,第4期
• 2. An Evaluation of the Selectivity Characteristics of Different Juvenile Fish Escape Panel Designs for the Demersal Trap Fishery of Abu Dhabi, United Arab Emirates [J] . Edwin Mark Grandcourt ,Thabit Zahran Al Abdessalaam ,Stanley Alexander Hartmann . 海洋科学期刊（英文） . 2011,第3期
• 3. 中华通草蛉卵低温存活力和幼虫耐饥力的初步研究 [C] . 曹艳霞 ,郅伦山 . 中国昆虫学会第八次全国会员代表大会暨2007年学术年会 . 2007
• 4. 四种瓢虫对草地贪夜蛾卵和幼虫的捕食功能研究 [A] . 孔琳 . 2020