首页>
外国专利>
METHOD FOR DISCRIMINATION OF INDIVIDUALS INFECTED WITH LYMPHOCYSTIS DISEASE VIRUS OR PARALICHTHYS OLIVACEUS INDIVIDUALS RESISTANT AGAINST LYMPHOCYSTIS DISEASE VIRUS
METHOD FOR DISCRIMINATION OF INDIVIDUALS INFECTED WITH LYMPHOCYSTIS DISEASE VIRUS OR PARALICHTHYS OLIVACEUS INDIVIDUALS RESISTANT AGAINST LYMPHOCYSTIS DISEASE VIRUS
展开▼
机译：鉴别抗淋巴结病病毒或抗食性圆斑病的个人的抗淋巴结病病毒的方法
展开▼
页面导航
摘要
著录项
相似文献
摘要
A method for discrimination of individuals infected with lymphocystis disease virus(LCDV) or Paralichthys olivaceus individuals having resistance against lymphocystis disease virus is provided to produce of healthy individuals without virus infection, reduce lymphocystis disease occurrence rate by treating lymphocystis disease at an early stage, and selectively produce the excellent Paralichthys olivaceus individuals. A genetic marker for discrimination of fish individuals infected with lymphocystis disease virus has the nucleotide sequence of SEQ ID NO:10, wherein the genetic marker is amplified by a pair of primers having the nucleotide sequences of SEQ ID NO:1 and SEQ ID NO:2, specific to NIF/NLI interacting factor gene; and the fish is Paralichthys olivaceus. The method for discrimination of fish individuals infected with lymphocystis disease virus comprises the steps of: (i) extracting the DNA sample of fish to be discriminated; (ii) amplifying the DNA sample with the primer pair of SEQ ID NO:1 and SEQ ID NO:2; and (iii) analyzing the amplification of the NIF/NLI interacting factor gene that is specific to the infection of lymphocystis disease virus.
展开▼
机译：提供了一种用于区分感染了淋巴囊肿病病毒（LCDV）的个体或具有对淋巴囊肿病病毒的抵抗力的橄榄寄生虫的个体的方法，以产生没有病毒感染的健康个体，通过早期治疗淋巴囊肿病来降低淋巴囊肿病的发生率，以及有选择地生产优秀的Paralichthys olivaceus个人。用于鉴别感染了淋巴囊虫病病毒的鱼个体的遗传标记具有核苷酸序列SEQ ID NO：10，其中该遗传标记通过一对具有核苷酸序列SEQ ID NO：1和SEQ ID NO：的引物进行扩增。 2，特异于NIF / NLI相互作用因子基因;鱼类是Paralichthys olivaceus。鉴别感染了淋巴囊病病毒的鱼类个体的方法包括以下步骤：（i）提取待鉴别鱼类的DNA样品; （ii）用SEQ ID NO：1和SEQ ID NO：2的引物对扩增DNA样品; （iii）分析特异性针对淋巴囊病病毒感染的NIF / NLI相互作用因子基因的扩增。
展开▼
