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METHOD FOR DETECTING METHYLAION OF PROMOTER USING RESTRICTION ENZYME AND DNA CHIP

机译：限制性内切酶和DNA芯片检测启动子甲基的方法

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摘要

The present invention relates to a method using the Hpa II restriction enzyme sensitive to methylation of detecting the methylation or absence of promoters, and more particularly, cutting a clinical sample or diagnosed origin of the DNA with restriction enzymes Hpa II, and the CpG island was amplified by PCR using primers designed to amplify the following, the present invention relates to PCR amplification the presence or absence of water in the method for detecting the methylation of the promoter, characterized in that whether to make a DNA chip for detecting methyl illustration. ; The invention may be useful in disease diagnosis, such as it can detect the promoter methylation of the genes to other simple, and economical in the conventional method, the promoter methylation occurring cancer. ; Detecting methylation, CpG island, HpaII, promoter hypermethylation

机译：本发明涉及使用对甲基化敏感的 Hpa II限制酶来检测启动子的甲基化或不存在启动子的方法，更具体地涉及用限制酶切割临床样品或诊断的DNA来源的方法。 I> Hpa II，使用设计用于扩增以下内容的引物通过PCR扩增CpG岛，本发明涉及在检测启动子甲基化的方法中PCR扩增水存在与否的方法，其特征在于在于是否制造用于检测甲基插图的DNA芯片。 ;本发明在疾病诊断中可能是有用的，例如它可以将基因的启动子甲基化检测到其他简单且经济的常规方法中，该启动子甲基化发生癌症。 ;检测甲基化，CpG岛，HpaII，启动子高甲基化 展开▼

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公开/公告号KR20060026595A

专利类型

公开/公告日2006-03-24

原文格式PDF

申请/专利权人 GENOMICTREE INC.;
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申请/专利号KR20040075395

发明设计人 LEE SUN WOO;WOO SUK KYUNG;OH TAE JEONG;YOON DAE KYOUNG;KIM MYUNG SOON;YOON CHI WANG;AN SUNG WHAN;
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申请日2004-09-21

分类号C12Q1/68;

国家 KR

入库时间 2022-08-21 21:26:13

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