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METHODS FOR IDENTIFYING INHIBITORS OF STEROL 14-alpha-DEMETHYLASE
METHODS FOR IDENTIFYING INHIBITORS OF STEROL 14-alpha-DEMETHYLASE
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机译:识别甾醇14-α-二甲基化酶抑制剂的方法
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摘要
In the methods of the present invention, the change in absorbance of an obtusifoliol 14a-demethylase (OBT-DM) enzyme upon binding of an inhibitor is used to advantage for the identification of inhibitors of OBT-DM activity. Rather than measuring the absorbance of an OBT-DM/inhibitor complex over a spectrum of wavelengths as described previously, the present invention discloses methods for identifying type II inhibitors of OBT-DM by monitoring the absorbance only at 413nm and 432 nm. The methods of the invention enable the concurrent testing of multiple compounds using a high throughput format such as with 96- or 384-well plates. The OBT-DM polypeptides of the invention include plant, fungal and human OBT-DM polypeptides, and in particular, Arabidopsis thaliana OBT-DM polypeptide.
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