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METHODS FOR IDENTIFYING INHIBITORS OF STEROL 14-alpha-DEMETHYLASE

机译:识别甾醇14-α-二甲基化酶抑制剂的方法

摘要

In the methods of the present invention, the change in absorbance of an obtusifoliol 14a-demethylase (OBT-DM) enzyme upon binding of an inhibitor is used to advantage for the identification of inhibitors of OBT-DM activity. Rather than measuring the absorbance of an OBT-DM/inhibitor complex over a spectrum of wavelengths as described previously, the present invention discloses methods for identifying type II inhibitors of OBT-DM by monitoring the absorbance only at 413nm and 432 nm. The methods of the invention enable the concurrent testing of multiple compounds using a high throughput format such as with 96- or 384-well plates. The OBT-DM polypeptides of the invention include plant, fungal and human OBT-DM polypeptides, and in particular, Arabidopsis thaliana OBT-DM polypeptide.
机译:在本发明的方法中,利用抑制剂结合后奥布斯福尔14a-脱甲基酶(OBT-DM)酶的吸光度变化有利于鉴定OBT-DM活性的抑制剂。本发明公开了通过仅在413nm和432nm处监测吸光度来鉴定OBT-DM的II型抑制剂的方法,而不是如前所述在波长范围内测量OBT-DM /抑制剂复合物的吸光度。本发明的方法能够使用高通量形式(例如96孔或384孔板)同时测试多种化合物。本发明的OBT-DM多肽包括植物,真菌和人的OBT-DM多肽,特别是拟南芥OBT-DM多肽。

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