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Site specific incorporation of heavy atom-containing unnatural amino acids into proteins for crystal structure determination
Site specific incorporation of heavy atom-containing unnatural amino acids into proteins for crystal structure determination
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机译：将含有重原子的非天然氨基酸定点结合到蛋白质中以测定晶体结构
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摘要
Translation systems and other compositions including orthogonal aminoacyl tRNA-synthetases that preferentially charge an orthogonal tRNA with an iodinated or brominated amino acid are provided. Nucleic acids encoding such synthetases are also described, as are methods and kits for producing proteins including heavy atom-containing amino acids, e.g., brominated or iodinated amino acids. Methods of determining the structure of a protein, e.g., a protein into which a heavy atom has been site-specifically incorporated through use of an orthogonal tRNA/aminoacyl tRNA-synthetase pair, are also described.
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