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RECOMBINANT MONO AND POLY ANTIGENS TO DETECT CYTOMEGALOVIRUS-SPECIFIC IGM IN HUMAN SERA BY ENZYME IMMUNOASSAY
RECOMBINANT MONO AND POLY ANTIGENS TO DETECT CYTOMEGALOVIRUS-SPECIFIC IGM IN HUMAN SERA BY ENZYME IMMUNOASSAY
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机译：重组酶和多抗原检测酶联免疫吸附法检测人血清中的人乳巨细胞病毒
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摘要
A mixture of recombinant mono- and poly-epitope proteic materials able tofully replace the viral antigens when used in an enzymeimmunoassay (EIA) is disclosed; the mixture includes a poly-epitope fusionprotein having a first region formed by an amino acid sequence(H10) corresponding to that of the last 233 amino acids of the COOH terminusof the vital protein p52 or to a part thereof, a secondregion formed by an amino acid sequence (F3) corresponding to that of the last43 amino acids of the COOH terminus of viral proteinpp150 or to a part thereof, and a third region formed by an amino acidsequence (AlC2) corresponding to that taken from as 595 to aa614, proceeding in direction 5'.fwdarw.3', of the same viral protein pp150;and, in combination, a second fusion protein including a sequenceof amino acids corresponding to that taken, proceeding in direction5'.fwdarw.3' from as 297 to aa 510 of the viral major matrix protein pp65encoded by the viral gene UL83 and a third fusion protein including a sequenceof amino acids corresponding to that taken, proceeding indirection 5'.fwdarw.3', from as 117 to as 373 of the vital assembly proteinpp38 encoded by the viral gene UL80a. These three fusion proteinsmay be used combined together for the preparation of an ELISA test kit fordetection of Cytomegalovirus-specific Igm in human sera.
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