首页>
外国专利>
Synthesis and complete chemical synthesis of genes genomes.
Synthesis and complete chemical synthesis of genes genomes.
展开▼
机译：基因组的合成和完全化学合成。
展开▼
页面导航
摘要
著录项
相似文献
摘要
A method of synthesizing a double-stranded polynucleotide capable of replication without using any recombinant DNA or existing natural degeneracy, wherein said polynucleotide comprises an origin of replication, a first coding region and a first regulatory element directing the expression of said first coding region, comprising the steps of: a) generating a first set corresponding to the entire plus strand of said double-stranded oligonucleotide oligonucleotides; b) generating a second set of oligonucleotides corresponding to the entire minus strand of said double-stranded oligonucleotide and c) annealing said first and second oligonucleotide sets; wherein each of said oligonucleotides of said second set of oligonucleotides overlaps with and hybridizes to two complementary oligonucleotides of said first set of oligonucleotides, while two oligonucleotides at a 5 'or 3'' of said oligonucleotide double-stranded hybridize I consolo a complementary oligonucleotide.
展开▼
