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Arrangement for binding molecules, e.g. useful in fluorescence microscopy studies, comprises individual functional groups or multiple identical functional groups arranged on a solid support at a defined density
Arrangement for binding molecules, e.g. useful in fluorescence microscopy studies, comprises individual functional groups or multiple identical functional groups arranged on a solid support at a defined density
Arrangement for binding molecules comprises individual functional groups or multiple identical functional groups arranged on a solid support at a density of 10 4-10 10functional groups per cm 2such that there are no further functional groups within a predetermined radial distance from any given functional group in at least 95% of cases. Independent claims are also included for: (1) producing an arrangement for binding individual molecules by providing a solid surface with functional groups, contacting the surface with auxiliary structures having units (especially organic groups, nucleic acids or polypeptides) capable of binding to the functional groups, treating the surface with agents that either inactivate functional groups not bound to auxiliary structures or block units not bound to functional groups, treating the surface with agents that cleave the linkage between the auxiliary structures and the bound units or fragments thereof, and removing the cleaved auxiliary structures; (2) producing an arrangement for binding individual molecules by providing a solid surface with functional groups and contacting the surface with auxiliary structures having units with activatable functional groups that can bind to the surface functional groups when activated by external stimuli, or providing a solid surface with activatable functional groups and contacting the surface with auxiliary structures having units with functional groups, applying an external stimulus to the surface so that the activated functional groups bind to the other functional groups, treating the surface with agents that cleave the linkage between the auxiliary structures and the bound units or fragments thereof, and removing the cleaved auxiliary structures; (3) producing an arrangement for binding individual molecules by applying nanoscopic islets onto the surface of a solid substrate by a surface structuring method, especially scanning tunneling microscopy, dip pen nanolithography, electron beam lithography, ion beam lithography or microcontact printing, applying adapters to the islets, coupling individual molecules to the adapters and blocking any unoccupied islets with adapter variants not bearing molecules; (4) producing an arrangement for binding individual molecules by applying nanoscopic islets onto the surface of a solid substrate by a surface structuring method, especially scanning tunneling microscopy, dip pen nanolithography, electron beam lithography, ion beam lithography or microcontact printing, coupling molecules to adapters in solution, removing uncoupled adapters or uncoupled molecules by purification steps and applying the molecule-coupled adapters to the substrate.
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