首页> 外国专利> Probes for target nucleic acid sequence (TS) and their application, a method of standard peptide synthesis technique, methods of detecting TS in dsNA, methods of detecting or amount identification of a given NA in a sample, method of amount identification of a given NA in a sample in real time, method of detecting TS in ssNA.

Probes for target nucleic acid sequence (TS) and their application, a method of standard peptide synthesis technique, methods of detecting TS in dsNA, methods of detecting or amount identification of a given NA in a sample, method of amount identification of a given NA in a sample in real time, method of detecting TS in ssNA.

机译：靶核酸序列（TS）的探针及其应用，标准肽合成技术的方法，dsNA中检测TS的方法，样品中给定NA的检测或量鉴定方法，给定NA的量鉴定方法在样本中实时检测ssNA中TS的方法。

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摘要

1. Probes for target nucleic acid sequence (TS) and their application, a method of standard peptide synthesis technique, methods of detecting TS in dsNA, methods of detecting or amount identification of a given NA in a sample, a method of amount identification of a given NA in a sample in real time, a method of detecting TS in ssNA, considerably modified when it binds with a nucleic acid (NA), comprising the above-mentioned target sequence (TS). At least in the part close to RG, the probe has a structure, which ensures that each intramolecular interaction between SRE and RG which has impact on the RG signal is inhibited, or it has a sequence of bases - at least at the end close to RG - which has minimum impact on the properties of the RG signal. 2. The probe identified in patent claim 1. It is characterised in that when binding with TS, it establishes a structure, which increases the signal change triggered by the binding of RG to NA. 3. The probe identified in patent claim 1. It is characterised in that at least in the part close to RG, SRE has a structure, which differs from the natural nucleic acid. 4. The probe identified in patent claim 1. it is characterised in that SRE is selected from a group comprising: a synthetic analogue of deoxyribonucleic acid (NAA), a sequence-recognising protein or peptide, an analogue of deoxyribonucleic acid bound with the protein or peptide, and an oligonucleotide bound with the protein or peptide.

机译：1.靶核酸序列（TS）的探针及其应用，标准肽合成技术的方法，dsNA中TS的检测方法，样品中给定NA的检测或量鉴定方法，样品中给定NA的量鉴定方法实时提供样品中给定的NA，一种检测ssNA中TS的方法，当它与包含上述目标序列（TS）的核酸（NA）结合时，其方法会大大改变。该探针至少在接近RG的部分具有一种结构，该结构可确保抑制SRE和RG之间对RG信号产生影响的每个分子内相互作用，或者具有一系列碱基-至少在接近RG的末端RG-对RG信号的特性影响最小。 2.根据权利要求1所述的探针，其特征在于，当与TS结合时，其建立一种结构，该结构增加了由RG与NA的结合触发的信号变化。 3.根据权利要求1所述的探针，其特征在于，至少在接近RG的部分中，SRE具有与天然核酸不同的结构。 4.权利要求1中鉴定的探针，其特征在于SRE选自：脱氧核糖核酸（NAA）的合成类似物，序列识别蛋白质或肽，与该蛋白质结合的脱氧核糖核酸的类似物。或肽，以及与蛋白质或肽结合的寡核苷酸。

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公开/公告号PL190263B1

专利类型
公开/公告日2005-11-30

原文格式PDF
申请/专利权人 LIGHTUP TECHNOLOGIES AB;
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申请/专利号PL19970330201
发明设计人 KUBISTA MIKAEL;SVANVIK NICKE;
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申请日1997-05-30
分类号C12N15/11;C07D417/06;C07H21/00;C12Q1/68;
国家 PL
入库时间 2022-08-21 21:37:59

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