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METHOD FOR CONVEYING RESISTANCE TO BEET NECROTIC YELLOW VEIN VIRUS (BNYVV), THE USE DNA VECTOR THEREFOR AND A PLANT AND A CELL THEREOF RESISTANT AGAINST SAID VIRUS
METHOD FOR CONVEYING RESISTANCE TO BEET NECROTIC YELLOW VEIN VIRUS (BNYVV), THE USE DNA VECTOR THEREFOR AND A PLANT AND A CELL THEREOF RESISTANT AGAINST SAID VIRUS
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机译：对抗甜菜黄性黄脉病毒（BNYVV）的抗性的方法，使用DNA矢量载体和植物以及抗所述病毒的细胞
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摘要
The present invention relates to a method for conveying to a sugar beet plant resistance to beet necrotic yellow vein virus (BNYVV), comprising the following steps:(a) preparing a DNA fragment of at least 15 nucleotides in a sequence that is essentially homologous to the corresponding nucleotide sequence of the genomic RNA 1 of the beet necrotic yellow vein virus (BNYVV), said fragment comprises at least a part of open frame of helicase reading;(b) introducing said DNA fragment, operatively linked to a promotor that is active in sugar beet plants, into a sugar beet plant cell to obtain a transformed sugar beet cell; and(c) regenerating a transgenic sugar beet plant from the transformed sugar beet plant cell.In addition, the invention relates to a DNA vector harboring a fragment of a least 15 nucleotides in a sequence that is essentially homologous to the corresponding nucleotide sequence of the genomic RNA 1 of BNYVV, said fragment comprises at least a part of open frame helicase reading and transcription and translation regulatory sequences operably linked therewith, as well as to the use of said DNA vector for the transformation of a plant cell to obtain plants that are resistant against BNYVV. The invention further relates to a sugar beet plant cell resistant against BNYVV comprising in its genome a DNA fragment and the use of said plant cell for the regeneration therefrom of a sugar beet plant that is resistant against BNYVV. The present invention also relates to progeny of a sugar beet plant and to seeds of a sugar beet plant or progeny. The present invention relates to vegetatively reproducible structures such as calluses, buds, embryos from a plant or progeny. The present invention relates to the use of said seeds for regenerating a sugar beet plant therefrom that are resistant against BNYVV, to the use of vegetatively reproducible structures for regenerating a sugar beet plant therefrom that are resistant against BNYVV.
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