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首页> 外国专利> methods for determining at least one substance production that affects metabolic flow using cells, for creating a bacterial strain having an ability to produce substance, and for producing a substance, program for determining at least one substance production that affects metabolic flow using cells, and computer readable registration medium

methods for determining at least one substance production that affects metabolic flow using cells, for creating a bacterial strain having an ability to produce substance, and for producing a substance, program for determining at least one substance production that affects metabolic flow using cells, and computer readable registration medium

机译：确定使用细胞影响代谢流动的至少一种物质产生的方法，产生具有产生物质能力的细菌菌株以及产生物质的方法，确定使用细胞影响代谢流动的至少一种物质产生的程序和计算机可读的注册媒体

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"METHODS FOR DETERMINING AT LEAST A SUBSTANCE PRODUCTION THAT AFFECTS METABOLIC FLOW USING CELLS, TO CREATE A BACTERIAL CUP, PRESENTING A CAPACITY TO PRODUCE SUBSTANCE, WHICH PRODUCE A SUBSTANCE PROGRAM MEASURES METABOLIC FLOW , AND RECORD MEANS THAT CAN BE READ BY COMPUTER ". Substance production that affects metabolic flow using cells is determined by 1) creating a stoichiometric matrix based on biochemical reactions formulas of a substrate by means of a desired produced substance, 2) selecting the same number of independent metabolic flows of all metabolic flows that the degree of freedom of the stoichiometric matrix as free flows, 3) creating a sufficient number of random free flow combinations for a statistical analysis and calculation of a metabolic flow distribution of each combination created based on the matrix. stoichiometric, 4) obtaining a regression equation including a minimum number of free flows showing a correlation with substance production of calculated metabolic flow distributions by multivariate statistical analysis, and 5) determining at least one substance production that affects flu metabolic rate based on a coefficient in the regression equation obtained.
机译：“一种用于确定至少一种影响使用细胞的代谢流的物质生产，创建细菌杯，呈现产生物质能力的方法，该物质程序可以测量代谢物流量，并据此进行记录。”影响细胞代谢活动的物质的确定方法是：1）通过所需产生的物质，基于底物的生化反应公式创建化学计量矩阵，2）在所有代谢流中选择相同数量的独立代谢流，其程度取决于3）创建足够数量的随机自由流组合用于统计分析和计算基于矩阵创建的每个组合的代谢流分布。化学计量; 4）通过多元统计分析获得包括与计算的代谢流量分布的物质产生相关的最小自由流量的回归方程，以及5）根据以下公式确定影响流感代谢率的至少一种物质：获得的回归方程。

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