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首页> 外国专利> Microbiological process for obtaining bacterial plasmu00ecdeos (vectors or expression cassettes) using vector with thermo sensitive open promoter derived from lambda phage and its application in obtenu00e7Will be high nu00ecveis secretion proteu00ecnas in space periplu00e1smico or in the cytoplasm of bacteria

Microbiological process for obtaining bacterial plasmu00ecdeos (vectors or expression cassettes) using vector with thermo sensitive open promoter derived from lambda phage and its application in obtenu00e7Will be high nu00ecveis secretion proteu00ecnas in space periplu00e1smico or in the cytoplasm of bacteria

机译：使用具有衍生自λ噬菌体的热敏开放启动子的载体获得细菌质体（载体或表达盒）的微生物学方法及其在肥胖中的应用将是空间周界或细胞质中高necucis分泌蛋白的表达。细菌

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Microbiological process for obtaining bacterial plasmu00ecdeos (vectors or expression cassettes) using vector with thermo sensitive open promoter derived from lambda phage and its application to obtainTion of high nu00ecveis secretion proteu00ecnas in space periplu00e1smico or in the cytoplasm of bacteria. The patent relates to the microbiological process to obtain a bacterial plasmid, open, highly efficient, used as a tape of expression (cassette expression vector '),Enabling to obtain other vectors, with more specific characteristics, such as: resistance against antibiotics and secretion of recombinant protein for the periplasma or bacterial cytoplasm.The condition that defined by the presence or not of the sequence signal, respectively.The process includes linearization and defosforilau00e7u00e3o of tape of expression after introduction of restriction sites ndel and bam hi and a spacer sequence specific and particularly effective.And the introduction of different signaling sequences for use in bacteria. The promoter is the lambda P ~ l ~ (Bernard, H.; helinski, D.R., methods enzymol.1979, 955: 229 236), whose activation occurs by temperature increase.One of the applications of the tape of expression is obtained hormu00f3nio plasmids for secretion of human growth and human prolactin.Confirming the efficiency of the system for expression of heterologous proteins in bacteria.
机译：使用具有λ噬菌体来源的热敏开放启动子的载体获得细菌质膜（载体或表达盒）的微生物学方法及其在空间周质或细菌细胞质中获得高necececis分泌蛋白的应用。该专利涉及获得表达载体（盒式表达载体'）的开放，高效的细菌质粒的微生物学过程，从而能够获得具有更具体特征的其他载体，例如：对抗生素和重组蛋白分泌到周质或细菌细胞质中的条件，分别由序列信号的存在与否所定义。该过程包括引入限制性位点ndel和bam hi后表达带的线性化和去fosforila u00e7 u00e3o。以及特异性和特别有效的间隔子序列。以及引入用于细菌的不同信号序列。启动子是λP〜l〜（Bernard，H .; helinski，DR，Methods enzymol.1979，955：229 236），其激活通过温度升高而发生。表达带的一种应用是获得激素。用于人类生长和催乳素分泌的u00f3nio质粒。证实了该系统在细菌中表达异源蛋白的效率。

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