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首页> 外国专利> Method for cryopreserving microencapsulated living animal cells enclosed in immunoisolation membranes, such microencapsulated living animal cells in immunoisolation membranes, and biohybrid artificial organ modules using such microencapsulated living animal cells in immunoisolation membranes

Method for cryopreserving microencapsulated living animal cells enclosed in immunoisolation membranes, such microencapsulated living animal cells in immunoisolation membranes, and biohybrid artificial organ modules using such microencapsulated living animal cells in immunoisolation membranes

机译：冷冻保存在免疫隔离膜中的微囊化的活体动物细胞，在免疫隔离膜中的微囊化的活体动物细胞以及在免疫隔离膜中使用这种微囊化的活体动物细胞的生物杂交人工器官模块的冷冻保存方法

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摘要

A method is disclosed for cryopreserving living animal cells in immunoisolation membranes, including: (1) cutting out a living organ from an animal, (2) digesting the cutout organ into the discrete living animal cells and separating the discrete cells, (3) suspending the separated cells in a solution of sodium chloride containing sodium alginate and collagen, (4) forming microcapsules of the living animal cells by using the resulting suspension, (5) forming immunoisolation membranes around outer surfaces of the microcapsules of the living animal cells by covering the outer surfaces with alginate-(poly-L-lysine) and thereby obtaining the living animal cells enclosed in the immunoisolation membranes, (6) suspending the resulting living animal cells enclosed in the immunocapsules in a cell damage-preventing solution, and (7) immediately with liquid nitrogen.
机译：公开了一种用于在免疫隔离膜中冷冻保存活体动物细胞的方法，该方法包括：（1）从动物中切出活体器官，（2）将切出的器官消化成离散的活体动物细胞并分离离散的细胞，（3）悬浮在含有海藻酸钠和胶原蛋白的氯化钠溶液中分离出的细胞，（4）通过使用所得的悬浮液形成活体动物细胞的微囊，（5）通过覆盖在活体动物细胞的微囊的外表面周围形成免疫隔离膜用藻酸盐-（聚-L-赖氨酸）的外表面获得封闭在免疫隔离膜中的活动物细胞，（6）将所得封闭在免疫胶囊中的活动物细胞悬浮在防止细胞损伤的溶液中，和（7 ）立即用液氮。

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