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MULTI-POINT FLUORESCENCE SPECTROPHOTOMETRY MICROSCOPE AND MULTI-POINT FLUORESCENCE SPECTROPHOTOMETRY METHOD

机译:多点荧光分光光度法和多点荧光分光光度法

摘要

PPROBLEM TO BE SOLVED: To provide a multi-point fluorescence spectrophotometry microscope and a multi-point fluorescence spectrophotometry method, by which an arbitrary measurement target area can be set easily on a specimen and the spectral information of each measurement target area can be accurately obtained. PSOLUTION: A DMD 3 is disposed in a position that is optically conjugate with the focused position of an objective lens 1, by which light from an illuminating optical system generating light from a light source is condensed on the specimen S. ROI on the specimen S is set from the imaging screen of a CCD camera 13 that images light generated from the specimen S. According to the ROI thus set, the reflection pattern of the DMD 3 is controlled. Light generated from an area, corresponding to the ROI on the specimen S is obtained by a grating 18 and a line photosensor array 19, by subjecting the light to spectrum spread. PCOPYRIGHT: (C)2006,JPO&NCIPI
机译:

要解决的问题:提供一种多点荧光分光光度法显微镜和多点荧光分光光度法,通过该方法可以轻松地在样品上设置任意测量目标区域,并且可以测量每个测量目标区域的光谱信息。准确获得。

解决方案:DMD 3放置在与物镜1的聚焦位置光学共轭的位置,通过它,来自产生光源的光的照明光学系统的光会聚在样品S上。从CCD照相机13的成像屏幕设置样本S,该CCD照相机13对从样本S产生的光进行成像。根据如此设置的ROI,控制DMD 3的反射图案。光栅18和线型光电传感器阵列19通过使光经受光谱扩展而获得从与样本S上的ROI相对应的区域产生的光。

版权:(C)2006,JPO&NCIPI

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