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EXCITATION SPECTRUM CORRECTION TECHNIQUE IN FLUORESCENCESPECTROPHOTOMETER
EXCITATION SPECTRUM CORRECTION TECHNIQUE IN FLUORESCENCESPECTROPHOTOMETER
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机译：荧光分光光度计中的激发光谱校正技术
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摘要
PROBLEM TO BE SOLVED: To provide a spectrum correction technique usable to a wide range of wavelength and also applicable to a fluorescencespectrophotometer, in which the variable range of wavelength of excitation spectrometer is differ greatly to the variable range of fluorescencespectrometer.;SOLUTION: Using a spectro-monitor B composed in centering around a spectroscope 15 with the wavelength extent including the wavelength extent of excitation spectrometer 2 of the fluorescencespectrophotometer A and a known tungsten standard lamp C with known wavelength strength property, firstly the wavelength characteristic of spectro-monitor B is derived from the spectrum of tungsten standard lamp C, and next, while measuring light from the excitation spectrometer 2 with the spectro-monitor B, the both is scanned synchronously and using the acquired spectrum the wavelength characteristic is derived of excitation system. Finally, by measuring the spectrum of tungsten standard lamp C with the fluorescencespectroscope 7 the wavelength characteristic of fluorescence system is derived.;COPYRIGHT: (C)2007,JPO&INPIT
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