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Preparing lyophilized composition containing nucleic acid, useful, after rehydration, for polymerase chain reaction amplification, with addition of disaccharide and collagen as stabilizers
Preparing lyophilized composition containing nucleic acid, useful, after rehydration, for polymerase chain reaction amplification, with addition of disaccharide and collagen as stabilizers
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机译：制备含有核酸的冻干组合物，在复水后可用于聚合酶链反应扩增，并添加二糖和胶原蛋白作为稳定剂
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摘要
Preparing a lyophilized composition (A) containing nucleic acid (NA), comprising lyophilizing an aqueous solution of NA in presence of a disaccharide (I) and collagen (II), is new. Independent claims are also included for the following: (1) (A) prepared by the new method; (2) lyophilized composition (A1) containing NA, (I), e.g. trehalose, and (II); and (3) amplifying NA comprising rehydration of (A) or (A1) with an aqueous solution that optionally contains one or more components required for amplification by PCR, especially a DNA matrix.
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