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Flow chamber for in-vitro investigation of cell surface processes under microscope, comprises seal and spring clamp holding culture dish

机译:用于在显微镜下体外研究细胞表面过程的流动室,包括密封件和固定培养皿的弹簧夹

摘要

A culture dish (7) surface, wetted by a cell culture, is located over a planar surface of the flow chamber (1). A seal (6) is located in a groove (3) surrounding the flow chamber, that projects up. The cell culture dish is fixed and sealed to the body of the flow chamber by the spring-loaded lever (5). A culture dish (7) surface, wetted by a cell culture, is located over a planar surface of the flow chamber (1). A seal (6) is located in a groove (3) surrounding the flow chamber, that projects up. The cell culture dish is fixed and sealed to the body of the flow chamber by the spring-loaded lever (5). The lever is resilient. Its arrangement on the flow chamber is flexurally-resistant. It is connected to it by a pivot (9). It is held on a spring mounting (10), a spring (4) exerting pressure on the lever. The spring is a: spiral spring, leaf spring, foam, rubber, resilient plastic, thermoplastic, or a combination. The spring force is 0.1 N to 20 N. The outer contour of the chamber corresponds with the inner contour of commercially-available cell culture dishes (2). The seal is fixed in the depression. It is molded-in, or is held by friction and detachable. Seal and recess dimensions are detailed. The flow chamber is 0.5-50 cm in height. The culture cell is interchangeable. An adaptor plate is included between dish (2) and lever, to distribute pressure uniformly.
机译:通过细胞培养液润湿的培养皿(7)表面位于流动室(1)的平面上方。密封件(6)位于围绕流动室的凹槽(3)中,该密封件向上突出。细胞培养皿通过弹簧杆(5)固定并密封在流通腔的主体上。通过细胞培养液润湿的培养皿(7)表面位于流动室(1)的平面上方。密封件(6)位于围绕流动室的凹槽(3)中,该密封件向上突出。细胞培养皿通过弹簧杆(5)固定并密封在流通腔的主体上。杠杆是有弹性的。它在流动腔上的布置是抗弯曲的。它通过枢轴(9)连接到它。它被固定在弹簧座(10)上,弹簧(4)在杠杆上施加压力。弹簧是:螺旋弹簧,板簧,泡沫,橡胶,弹性塑料,热塑性塑料或它们的组合。弹簧力为0.1 N到20N。腔室的外部轮廓与市售细胞培养皿(2)的内部轮廓相对应。密封件固定在凹陷处。它是模制成型的,或通过摩擦固定和可拆卸的。详细介绍了密封件和凹槽的尺寸。流动室的高度为0.5-50厘米。培养细胞是可互换的。碟形件(2)和杠杆之间包括一个转接板,以均匀地分配压力。

著录项

  • 公开/公告号DE10328277A1

    专利类型

  • 公开/公告日2005-01-13

    原文格式PDF

  • 申请/专利权人 RWTH AACHEN;

    申请/专利号DE2003128277

  • 申请日2003-06-23

  • 分类号C12M1/34;

  • 国家 DE

  • 入库时间 2022-08-21 22:01:21

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