METHOD FOR TREATING HEAVY CRANIOCEREBRAL INJURY CASES

• 1. 治疗重型颅脑损伤病人胃肠功能紊乱的中药助消贴 [P] . 中国专利： CN106075255A . 2016-11-09
• 2. 一种中药组合物在制备治疗急性重型颅脑损伤的药物中的应用 [P] . 中国专利： CN101564438B . 2012.05.30
• 3. METHOD FOR PROPHYLAXIS AND TREATING PURULENT PULMONARY COMPLICATIONS ASSOCIATED WITH HEAVY CRANIOCEREBRAL INJURY [P] . 俄罗斯专利： RU2089244C1 . 1997-09-10

  机译： 预防和治疗重型颅脑损伤所致的脓性肺部并发症的方法

• 4. METHOD OF TREATING SECONDARY IMMUNODEFICIENCY IN CHILDREN IN POST-OPERATIVE PERIOD IN CASE OF SEVERE CRANIOCEREBRAL INJURY, COMBINED WITH INJURIES OF MUSCULO-SCKELETAL SYSTEM [P] . 俄罗斯专利： RU2355419C2 . 2009-05-20

  机译： 重度颅脑损伤合并肌肉骨骼系统损伤的儿童手术后继发免疫缺陷的治疗方法

• 5. METHOD FOR CORRECTING HEMODYNAMIC DISORDERS IN HEAVY CRANIOCEREBRAL INJURY CASES [P] . 俄罗斯专利： RU2309664C1 . 2007-11-10

  机译： 纠正重型颅脑损伤病例中的血液动力学异常的方法

• 1. COMPARATIVE OBSERVATION ON CURATIVE EFFECT OF POSTOPERATIVE SYMPTOMS OF THE CLOSED CRANIOCEREBRAL INJURY TREATED BY ACUPUNCTURE [J] . 丁晶, 张存生 世界针灸杂志：英文版 . 1997,第003期
• 2. Effect of different surfactants on removal efficiency of heavy metals in sewage sludge treated by a novel method combining bio-acidification with Fenton oxidation [J] . 任苗苗, 林宁波, 袁兴中, 中南大学学报（英文版） . 2014,第012期
• 3. EIGHTY CASES of SOFT TISSUE INJURY TREATED WITH LOCAL CONTRALATERAL NEEDLING METHOD [J] . 世界针灸杂志：英文版 . 1995,第001期
• 4. Clinical features and prevention of acute encephalocele in severe craniocerebral injury surgery [C] . Yang Sun International Symposium on Biomedical Science, Biotechnology and Healthcare . 2018
• 5. Investigating the impact of diffuse axonal injury on working memory performance following traumatic brain injury using functional and diffusion neuroimaging methods [D] . Turner, Gary R. 2008
• 6. Effect of Organics on Heavy Metal-Contaminated River Sediment Treated with Electro-Osmosis and Solidification/Stabilization Methods [O] . Chonggen Pan, Keyu Chen, Danting Chen 2020
• 7. Clinical study on acute craniocerebral injury treated with mild hypothermia auxiliary therapy [O] . Cheng Zhang, Jian-Ming Li, De-Zhang Dou, 2016
• 8. Automated System for Diagnosing Craniocerebral Injury [R] . Moiseyeva, N. I., Luchko, G. D., Kremleva, R. V., 1980

• 1. BACKGROUND： Vibrio vulnifi cus inside the body could activate the NF-!B signaling pathwayand initiate the inflammatory cascade. The lung is one of the earliest organs affected by sepsisassociated with acute lung injury. High mobility group protein B1 （HMGB1） is an important late-actingpro-infl ammatory cytokine involving in the pathophysiology of sepsis. It is also involved in the injuryprocess in the lung, liver and intestine. There has been no report on the involvement of HMGB1 inVibrio vulnifi cus sepsis-induced lung injury.METHODS： Sixty rats were randomly divided into a normal control group （group A, n=10） anda Vibrio vulnificus sepsis group （group B, n=50）. Sepsis was induced in the rats by subcutaneousinjection of Vibrio vulnificus （concentration 6×108 cfu/mL, volume 0.1 mL/100g）） into the left lowerlimbs. The rats in group B were sacrifi ced separately 1, 6, 12, 24, and 48 hours after the infection.Their lungs were stored as specimens, lung water content was measured, and lung pathology wasobserved under a light microscope. The expressions of the HMGB1 gene and protein in the lungswere detected by RT-PCR and Western blot. Data were analyzed with one-way analysis of variance（ANOVA） and the LSD method for pair-wise comparison between the two groups. P〈0.05 wasconsidered statistically signifi cant.RESULTS： Compared to group A （0.652±0.177）, HMGB1 mRNA expression in the lungs ofgroup B was signifi cantly higher at 0 hour （1.161±0.358, P=0.013）, 24 hours （1.679±0.235, P=0.000）,and 48 hours （1.258±0.274, P=0.004） （P〈0.05）, and peaked at 24 hours. Compared to group A（0.594±0.190）, HMGB1 protein expression at 6 hours （1.408±0.567, P=0.026） after infection wassignificantly increased （P〈0. 05）, and peaked at 24 hours （2.415±1.064, P=0.000） after infection.Compared to group A （0.699±0.054）, lung water content was significantly increased at 6 hours（0.759±0.030, P=0.001）,12 hours （0.767±0.023, P=0.000）, 24 hours （0.771±0.043, P=0.000） and 48hours （0.789±0.137, P=0.000） after infection （P〈0.05）. Compared to group A, pathological changesat 12 hours in group B indicate marked pulmonary vascular congestion, interstitial edema andinfl ammatory infi ltration. Alveolar cavity collapse and boundaries of the alveolar septum could not beclearly identifi ed.CONCLUSION： Vibrio vulnifi cus sepsis can lead to injury in rat lungs, and increased HMGB1expression in lung tissue may be one of the mechanisms for injury from Vibrio vulnifi cus sepsis. [J] . Qiao-meng Qiu ,Zhong-wang Li ,Lu-ming Tang . 世界急诊医学杂志(英文) . 2011,第4期
• 2. Effect of different surfactants on removal efficiency of heavy metals in sewage sludge treated by a novel method combining bio-acidification with Fenton oxidation [J] . 任苗苗 ,袁兴中 ,朱艺 . 中南大学学报 . 2014,第012期
• 3. 标准大骨瓣开颅血肿清除联合急诊治疗重型颅脑损伤的治疗 [C] . 王坤 ,袁萍 ,李冰 . 青岛市第十五届学术年会 . 2017
• 4. 中西医结合治疗急性重型颅脑损伤的综合治疗方案研究 [A] . 马朝晖 . 2007