FIELD: biotechnology and pharmaceutical industry.;SUBSTANCE: title operations are accomplished by following way. Three-dimensional culture of stromal cells is cultured in piston flow bioreactor, in particular being introduced in fibrous matrix incorporated into substrate, which is placed in container constituting a part of bioreactor piston. Stromal cells are grown until density 5 x 106 cell/cm3 substrate is attained, whereupon non-differentiated hemopoietic cells are either sowed directly into piston flow bioreactor or cultured in conditioned medium of stromal cells obtained by gathering medium from indicated flow bioreactor. Non-differentiated hemopoietic cells obtained by cultivation in presence of three-dimensional culture of stromal cells or their conditioned medium are transplanted to into recipient.;EFFECT: enabled growth of large amounts of stromal cells within a relatively small volume to provide longer maintenance of vital activity and reproduction of non-differentiated hemopoietic stem cells or precursor cells.;77 cl, 9 dwg, 3 tbl
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机译:领域:生物技术和制药工业;实体:标题操作通过以下方式完成。在活塞流生物反应器中培养基质细胞的三维培养物,特别是将其引入并入基质的纤维基质中,该基质被置于构成生物反应器活塞一部分的容器中。基质细胞生长直至密度达到5 x 10 6 Sup>细胞/ cm 3 Sup>底物,然后将未分化的造血细胞直接播种到活塞流生物反应器中或在条件下培养基质细胞的培养基,该培养基是通过从指示的流式生物反应器中收集培养基而获得的。通过在基质细胞的三维培养或其条件培养基的存在下培养获得的未分化造血细胞移植到受体中;效果:能够在相对较小的体积内大量基质细胞的生长,以提供对生命的更长的维持未分化造血干细胞或前体细胞的活性和繁殖。; 77 cl,9 dwg,3 tbl
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