首页> 外国专利> RECOMBINANT PLASMID DNA PCDNA-TCI PROVIDING EXPRESSION OF ARTIFICIAL GENE TCI IN EUCARYOTIC CELLS AND RECOMBINANT ATTENUATED STRAIN OF BACTERIUM SALMONELLA ENTERITIDIS E-23 PC-DNA-TCI AS CANDIDATE FOR CONSTRUCTION OF LIVE DNA-VACCINE AGAINST HUMAN IMMUNODEFICIENCY VIRUS

RECOMBINANT PLASMID DNA PCDNA-TCI PROVIDING EXPRESSION OF ARTIFICIAL GENE TCI IN EUCARYOTIC CELLS AND RECOMBINANT ATTENUATED STRAIN OF BACTERIUM SALMONELLA ENTERITIDIS E-23 PC-DNA-TCI AS CANDIDATE FOR CONSTRUCTION OF LIVE DNA-VACCINE AGAINST HUMAN IMMUNODEFICIENCY VIRUS

机译：重组质粒DNA PCDNA-TCI提供人基因TCI在抗癌细胞中的表达以及重组减毒的肠炎沙门氏菌E-23 PC-DNA-TCI菌株，作为构建针对人免疫缺陷病毒的活DNA疫苗的候选抗体

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FIELD: biotechnology, genetic engineering, molecular biology.;SUBSTANCE: invention proposes recombinant DNA pcDNA-TCI that has molecular mass 4.3 x 10³kDa and size 6 657 nucleotide pairs. The proposed recombinant plasmid DNA provides expression of artificial gene TCI comprising cytotoxic T-cellular epitopes of HIV-1 in eucaryotic cells. Also, invention proposes recombinant attenuated strain of bacterium Salmonella enteritidis E-23 pcDNA-TCI. The proposed strain is obtained by genetic transformation of the strain Salmonella enteritidis E-23 with plasmid pcDNA-TCI. The strain provides delivery DNA-vaccine in eucaryotic cells as the recombinant plasmid DNA pcDNA-TCI. Proposed groups of inventions provide inducing the expressed specific humoral and cellular immune response to HIV-1 in body. Proposed group of inventions can be used in medicine and virology for construction of live DNA-vaccine against HIV-1.;EFFECT: valuable biological and medicinal properties of strain and vaccine.;2 cl, 3 dwg, 3 tbl, 5 ex

机译：领域：发明提出了重组DNA pcDNA-TCI，其分子量为4.3 x 10 ^{3 kDa，大小为6 657个核苷酸对。所提出的重组质粒DNA在真核细胞中提供了包含HIV-1的细胞毒性T细胞表位的人工基因TCI的表达。另外，本发明提出了肠炎沙门氏菌E-23 pcDNA-TCI的重组减毒株。通过用质粒pcDNA-TCI对肠炎沙门氏菌E-23菌株进行遗传转化获得了所提出的菌株。该菌株以重组质粒DNA pcDNA-TCI的形式在真核细胞中提供递送DNA疫苗。提出的一组发明提供了诱导表达的针对人体中HIV-1的特定体液和细胞免疫应答。提议的一组发明可以在医学和病毒学领域用于构建抗HIV-1的活DNA疫苗;效果：菌株和疫苗的宝贵生物学和医学特性; 2 cl，3 dwg，3 tbl，5 ex}

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公开/公告号RU2248396C2

专利类型
公开/公告日2005-03-20

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申请/专利号RU20030111095
发明设计人 AGAFONOV A.P.;BELAVIN P.A.;SANDAKHCHIEV L.S.;VOROBEV A.A.;KARPENKO L.I.;NEKRASOVA N.A.;ILICHEV A.A.;BAZHAN S.I.;DANILJUK N.K.;BABKINA I.N.;IGNATEV G.M.;BOJCHENKO M.N.;SEREGIN S.V.;
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申请日2003-04-17
分类号C12N15/48;C12N15/63;A61K39/21;A61K39/112;C12N1/21;C12N1/21;C12R1/42;
国家 RU
入库时间 2022-08-21 22:02:08

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