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The Quantification Method of the Manila clam, Ruditapes philippinarum, egg protein using immunological Technique
The Quantification Method of the Manila clam, Ruditapes philippinarum, egg protein using immunological Technique
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机译:马尼拉蛤,菲律宾蛤仔,蛋蛋白的免疫学定量方法
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摘要
The invention antigen- to techniques for quantitation and further quantify the production of food clam a clam I protein by developing antibodies to the animals dissolved overdue using the principle of the antibody response clam, I of Ruditapes philippinarum protein. More particularly, the polyclonal antibody (polyclonal antibody) is one to be formed, and then cross-reaction with the immune adsorption with antibodies clam body tissues (Somatic tissue) of the clam eggs in the rabbit vivo by injecting the purified clam eggs extract the rabbit remove and specific binding enzyme to separate only the antibodies that react with immune adsorption method (enzyme Linked Immunosorbent Assay, ELISA) for use gonad of clam with measuring nandanbaek weight of the clam and this by selectively detect only the clam seen in clam tissue to clam al the index (somatic Gonadal index, GSI = gonadal weight / whole clam meat weight) relates to a technology for quantitatively measured.
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