首页> 外国专利> The Quantification Method of the Manila clam, Ruditapes philippinarum, egg protein using immunological Technique

The Quantification Method of the Manila clam, Ruditapes philippinarum, egg protein using immunological Technique

机译:马尼拉蛤,菲律宾蛤仔,蛋蛋白的免疫学定量方法

摘要

The invention antigen- to techniques for quantitation and further quantify the production of food clam a clam I protein by developing antibodies to the animals dissolved overdue using the principle of the antibody response clam, I of Ruditapes philippinarum protein. More particularly, the polyclonal antibody (polyclonal antibody) is one to be formed, and then cross-reaction with the immune adsorption with antibodies clam body tissues (Somatic tissue) of the clam eggs in the rabbit vivo by injecting the purified clam eggs extract the rabbit remove and specific binding enzyme to separate only the antibodies that react with immune adsorption method (enzyme Linked Immunosorbent Assay, ELISA) for use gonad of clam with measuring nandanbaek weight of the clam and this by selectively detect only the clam seen in clam tissue to clam al the index (somatic Gonadal index, GSI = gonadal weight / whole clam meat weight) relates to a technology for quantitatively measured.
机译:本发明涉及抗原的技术,其通过使用抗体反应蛤I的原理开发出针对逾期溶解的动物的抗体,从而定量定量蛤I蛋白的产量。更具体地讲,多克隆抗体(多克隆抗体)是一种待形成的抗体,然后通过注射纯化的蛤eggs卵提取物与兔体内蛤eggs卵的抗体蛤body体组织(体组织)的免疫吸附发生交叉反应。兔去除和特异性结合酶以仅分离与免疫吸附法反应的抗体(酶联免疫吸附测定,ELISA),以使用蛤的性腺并测量蛤dan的nandanbaek重量,并通过仅检测蛤only组织中可见的蛤to来检测蛤to的重量。蛤指数(体细胞性腺指数,GSI =性腺重量/蛤全肉重量)涉及一种定量测量的技术。

著录项

  • 公开/公告号KR100463456B1

    专利类型

  • 公开/公告日2005-01-07

    原文格式PDF

  • 申请/专利权人

    申请/专利号KR20010063573

  • 申请日2001-10-16

  • 分类号G01N33/577;

  • 国家 KR

  • 入库时间 2022-08-21 22:04:15

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