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HRP(HORSERADISH PEROXIDASE) CONJUGATED CARASSIUS AURATUS VITELLOGENIN MONOCLONAL ANTIBODY AND METHOD FOR MEASURING VITELLOGENIN USING THE SAME WITH REDUCED TIME AND IMPROVED ACCURACY
HRP(HORSERADISH PEROXIDASE) CONJUGATED CARASSIUS AURATUS VITELLOGENIN MONOCLONAL ANTIBODY AND METHOD FOR MEASURING VITELLOGENIN USING THE SAME WITH REDUCED TIME AND IMPROVED ACCURACY
PURPOSE: A HRP(horseradish peroxidase) conjugated Carassius auratus vitellogenin monoclonal antibody and a method for measuring vitellogenin using the same antibody are provided, thereby rapidly measuring vitellogenin and improving the measuring accuracy through inhibition of the nonspecific binding by directly binding a second monoclonal antibody coupling with the enzyme with the vitellogenin antigen. CONSTITUTION: The HRP(horseradish peroxidase) conjugated Carassius auratus vitellogenin monoclonal antibody is produced from a hybridoma cell line CVmA2(KCTC 10613BP). The method for measuring vitellogenin comprises the steps of: binding a vitellogenin antigen on the surface of a micro well plate coupling with a first Carassius auratus vitellogenin antibody; directly binding a second HRP conjugated Carassius auratus vitellogenin monoclonal antibody to the vitellogenin antigen; and inserting a test sample into the micro well plate.
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