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CHIMERIC CLONE OF HIV-1 SUBTYPE B FROM KOREAN WHICH IS USEFUL FOR DEVELOPMENT OF HIV(HUMAN IMMUNODEFICIENCY VIRUS) TREATING DRUGS AND HIV VACCINES SPECIFIC TO KOREAN AND METHOD FOR PREPARATION THEREOF
CHIMERIC CLONE OF HIV-1 SUBTYPE B FROM KOREAN WHICH IS USEFUL FOR DEVELOPMENT OF HIV(HUMAN IMMUNODEFICIENCY VIRUS) TREATING DRUGS AND HIV VACCINES SPECIFIC TO KOREAN AND METHOD FOR PREPARATION THEREOF
PURPOSE: A chimeric clone of HIV-1 subtype B from Korean and a method for preparation thereof are provided, which chimeric clone is useful for development of HIV(Human Immunodeficiency Virus) treating drugs and HIV vaccines specific to Korean. CONSTITUTION: The method for preparing the chimeric clone of HIV-1 subtype B from Korean comprises the steps of: isolating the genomic DNA of HIV-1 subtype B from Korean; PCR amplifying the genomic DNA of HIV-1 subtype B from Korean to obtain a 8,800 bp of PCR product; digesting HIV infectious clone pNL4-3 with EcoRI and XhoI, subjecting the digested pNL4-3 to electrophoresis on agarose gel, and isolating a 9.7 kb band instead of a 3 kb band containing HIV env gene; digesting the 8.8 kb of PCR product with EcoRI and XhoI to obtain a 3 kb fragment containing HIV-1 env gene; ligating the 9.7 kb of pNL4-3 with 3 kb fragment containing HIV-1 env gene using ligase; and transforming Escherichia coli with the ligation product, wherein the transformed E. coli is E. coli KRB 3050.1(KFCC-11333).
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