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COMPOSITION FOR SCREENING ANTIBIOTICS COMPRISING GENE SPR1373 FROM STREPTOCOCCUS PNEUMONIAE OR PROTEIN ENCODED THEREBY ESSENTIAL TO SURVIVAL OF STREPTOCOCCUS PNEUMONIAE, AND METHOD FOR SCREENING ANTIBIOTICS USING THE SAME
COMPOSITION FOR SCREENING ANTIBIOTICS COMPRISING GENE SPR1373 FROM STREPTOCOCCUS PNEUMONIAE OR PROTEIN ENCODED THEREBY ESSENTIAL TO SURVIVAL OF STREPTOCOCCUS PNEUMONIAE, AND METHOD FOR SCREENING ANTIBIOTICS USING THE SAME
PURPOSE: A composition for screening antibiotics comprising a gene spr1373 from Streptococcus pneumoniae or a protein encoded therein, and a method for screening antibiotics using the same are provided. The gene and protein encoded thereby are essential to survival of Streptococcus pneumoniae, and the antibiotics screening method against Streptococcus pneumoniae is useful for screening and developing antibiotics against other pathogenic bacteria. CONSTITUTION: The composition for screening antibiotics comprises a gene spr1373 from Streptococcus pneumoniae or a protein encoded thereby, wherein the gene spr1373 has the nucleotide sequence of SEQ ID NO:1, or the nucleotide sequence of SEQ ID NO: 1 wherein one or more mutations selected from disruption, deletion, insertion, point, substitution, nonsense, missense, polymorphism and rearrangement mutation are occurred; and the protein encoded by the gene spr1373 is UDP-N-acetylmuramate-alanine ligase having the amino acid sequence of SEQ ID NO:2 or a polypeptide fragment expressed from the nucleotide sequence of SEQ ID NO: 1 wherein one or more mutations selected from disruption, deletion, insertion, point, substitution, nonsense, missense, polymorphism and rearrangement mutation are occurred. The method for screening antibiotics comprises contacting the composition with a testing material, and determining whether the expression of gene spr1373 is inhibited.
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机译:目的:提供一种用于筛选抗生素的组合物,其包含来自肺炎链球菌的spr1373基因或其中编码的蛋白质,以及使用该组合物筛选抗生素的方法。由此编码的基因和蛋白质对于肺炎链球菌的生存是必不可少的,针对肺炎链球菌的抗生素筛选方法可用于筛选和开发针对其他病原细菌的抗生素。组成:用于筛选抗生素的组合物包含来自肺炎链球菌的spr1373基因或由其编码的蛋白质,其中该spr1373基因具有SEQ ID NO:1的核苷酸序列,或具有一个或多个突变的SEQ ID NO:1的核苷酸序列。从破坏,缺失,插入,点,取代,废话,错义,多态性和重排突变中选择;所述基因spr1373编码的蛋白质为具有SEQ ID NO:2的氨基酸序列或由SEQ ID NO:1的核苷酸序列表达的多肽片段的UDP-N-乙酰基村氨酸-丙氨酸连接酶,其中一个或多个突变选自发生破坏,缺失,插入,点,取代,废话,错义,多态性和重排突变。筛选抗生素的方法包括使组合物与测试材料接触,并确定基因spr1373的表达是否被抑制。
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