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PURIFICATION OF JOB'S TEARS EXTRACT AND PUMPKIN EXTRACT WHICH HAVE INHIBITORY EFFECTS ON AMYLASE AND LIPASE, RESPECTIVELY AND COMPOSITIONS CONTAINING JOB'S TEARS EXTRACT AND PUMPKIN EXTRACT FOR REGULATION OF OBESITY AND DIABETIC
PURIFICATION OF JOB'S TEARS EXTRACT AND PUMPKIN EXTRACT WHICH HAVE INHIBITORY EFFECTS ON AMYLASE AND LIPASE, RESPECTIVELY AND COMPOSITIONS CONTAINING JOB'S TEARS EXTRACT AND PUMPKIN EXTRACT FOR REGULATION OF OBESITY AND DIABETIC
PURPOSE: Provided are the purification of job's tears extract and pumpkin extract which have inhibitory effects on amylase and lipase, respectively and compositions for regulation of obesity and blood glucose level by using the same. Therefore, the compositions are used for improvement of obesity and diabetic. CONSTITUTION: The purification of job's tears extract comprises the steps of: extracting job's tear with hot water; concentrating the extract under reduced pressure, followed by centrifugation and ultrafiltration; subjecting the extract to sepharose Q FF ion exchange chromatography; and subjecting the extract to Sephadex G-200 gel chromatography. The purification of pumpkin extract omprises the steps of: extracting pumpkin with hot water; concentrating the extract and extracting the extract with ethylacetate; and subjecting the extract to silicagel column chromatography and RP-18 column chromatography.
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