Method for analysis of proteins by solution isoelectric focusing

• 1. 用于蛋白质组学分析的多肽混合物等电聚焦分离方法 [P] . 中国专利： CN103926301A . 2014-07-16
• 2. 一种用于分析水溶液中蛋白质二级结构的傅里叶变换红外光谱样品准备方法 [P] . 中国专利： CN106289919A . 2017-01-04
• 3. Method for analysis of proteins by solution isoelectric focusing [P] . 欧洲知识产权局专利： EP1564550B1 . 2010-10-20

  机译： 溶液等电聚焦分析蛋白质的方法

• 4. Method for analysis of proteins by solution isoelectric focusing [P] . 欧洲知识产权局专利： EP1564550A3 . 2005-08-24

  机译： 溶液等电聚焦分析蛋白质的方法

• 5. Method of capillary isoelectric focusing of proteins and peptides with fraction collection for post-run analysis [P] . 欧洲知识产权局专利： EP0617048A1 . 1994-09-28

  机译： 等分收集蛋白质和多肽的毛细管等电聚焦方法，用于运行后分析

• 1. Direct MALDI-MS Analysis of Proteins Isolated by Liquidphase Isoelectric Focusing Electrophoresis [J] . LIU Ning, L(U¨) Lei, ZHANG Xuan, 高等学校化学研究（英文版） . 2005,第004期
• 2. EXPLICATION OF CLINICAL EFFECTS OF ACUPUNCTURER BY ADAPTING MULTIVARIATE STATISTICAL ANALYSIS AND IMAGE-TECHNOLOGICAL METHODS: FOCUSING ON THE RELATIONSHIP WITH STRESS-RELATED DISEASES [J] . Kazu Mori 世界针灸杂志：英文版 . 1992,第004期
• 3. DIRECT MALDI-MS ANALYSIS OF PROTEINS ISOLATED BY LIQUID-PHASE ISOELECTRIC FOCUSING ELECTROPHORESIS [C] . . 2005
• 4. Integration of oxygen-18 labeling and solution isoelectric focusing in a shotgun analysis of mitochondrial proteins [D] . Wang, Jinshan. 2007
• 5. Integration of 18O Labeling and Solution Isoelectric Focusing in a Shotgun Analysis of Mitochondrial Proteins [O] . Jinshan Wang, Peter Gutierrez, Nathan Edwards, 2015
• 6. Electrophoresis and Isoelectric Focusing of Whole Cell and Membrane Proteins from the Extremely Halophilic Archaebacteria [R] . Stan-Lotter, Helga, Lang, Frank J., Jr., Hochstein, Lawrence I. 1989

• 1. BACKGROUND： Vibrio vulnifi cus inside the body could activate the NF-!B signaling pathwayand initiate the inflammatory cascade. The lung is one of the earliest organs affected by sepsisassociated with acute lung injury. High mobility group protein B1 （HMGB1） is an important late-actingpro-infl ammatory cytokine involving in the pathophysiology of sepsis. It is also involved in the injuryprocess in the lung, liver and intestine. There has been no report on the involvement of HMGB1 inVibrio vulnifi cus sepsis-induced lung injury.METHODS： Sixty rats were randomly divided into a normal control group （group A, n=10） anda Vibrio vulnificus sepsis group （group B, n=50）. Sepsis was induced in the rats by subcutaneousinjection of Vibrio vulnificus （concentration 6×108 cfu/mL, volume 0.1 mL/100g）） into the left lowerlimbs. The rats in group B were sacrifi ced separately 1, 6, 12, 24, and 48 hours after the infection.Their lungs were stored as specimens, lung water content was measured, and lung pathology wasobserved under a light microscope. The expressions of the HMGB1 gene and protein in the lungswere detected by RT-PCR and Western blot. Data were analyzed with one-way analysis of variance（ANOVA） and the LSD method for pair-wise comparison between the two groups. P〈0.05 wasconsidered statistically signifi cant.RESULTS： Compared to group A （0.652±0.177）, HMGB1 mRNA expression in the lungs ofgroup B was signifi cantly higher at 0 hour （1.161±0.358, P=0.013）, 24 hours （1.679±0.235, P=0.000）,and 48 hours （1.258±0.274, P=0.004） （P〈0.05）, and peaked at 24 hours. Compared to group A（0.594±0.190）, HMGB1 protein expression at 6 hours （1.408±0.567, P=0.026） after infection wassignificantly increased （P〈0. 05）, and peaked at 24 hours （2.415±1.064, P=0.000） after infection.Compared to group A （0.699±0.054）, lung water content was significantly increased at 6 hours（0.759±0.030, P=0.001）,12 hours （0.767±0.023, P=0.000）, 24 hours （0.771±0.043, P=0.000） and 48hours （0.789±0.137, P=0.000） after infection （P〈0.05）. Compared to group A, pathological changesat 12 hours in group B indicate marked pulmonary vascular congestion, interstitial edema andinfl ammatory infi ltration. Alveolar cavity collapse and boundaries of the alveolar septum could not beclearly identifi ed.CONCLUSION： Vibrio vulnifi cus sepsis can lead to injury in rat lungs, and increased HMGB1expression in lung tissue may be one of the mechanisms for injury from Vibrio vulnifi cus sepsis. [J] . Qiao-meng Qiu ,Zhong-wang Li ,Lu-ming Tang . 世界急诊医学杂志(英文) . 2011,第4期
• 2. Dynamics of Structural-Inhomogeneous Laminate and Shell Mechanical Systems with Point Constraints and Focused Masses. Part 2. Statement of the Problem of Forced Oscillations, Methods of Solution, Computational Algorithm and Numerical Results [J] . Mirsaidov Mirziyod ,Safarov Ismoil Ibrokhimovich ,Teshaev Mukhsin Khudoyberdievich . 应用数学与应用物理（英文） . 2019,第11期
• 3. Geometrically Nonlinear Analysis of Structures Using Various Higher Order Solution Methods: A Comparative Analysis for Large Deformation [J] . Ali Maghami ,Farzad Shahabian ,Seyed Mahmoud Hosseini . 工程与科学中的计算机建模(英文) . 2019,第12期
• 4. PDMS/Glass芯片等电聚焦及化学发光检测用于蛋白质分析 [C] . 黄香宜 ,任吉存 . 第六届全国毛细管电泳及相关微分离分析学术报告会 . 2004
• 5. 一维固相pH梯度等电聚焦与生物质谱联用鉴定混合蛋白质的方法建立与应用 [A] . 蒙根 . 2005