首页> 外国专利> $i(IN VITRO) FORMATION OF CONGOPHILIC MALTESE-CROSS AMYLOID PLAQUES TO IDENTIFY ANTI-PLAQUE THERAPEUTICS FOR THE TREATMENT OF ALZHEIMER'S AND PRION DISEASES

$i(IN VITRO) FORMATION OF CONGOPHILIC MALTESE-CROSS AMYLOID PLAQUES TO IDENTIFY ANTI-PLAQUE THERAPEUTICS FOR THE TREATMENT OF ALZHEIMER'S AND PRION DISEASES

机译：$ i（体外）形成糊状的十字形淀粉斑以鉴定抗老年斑和PR病毒病的抗斑治疗药物

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摘要

Co-incubation of an amyloid protein with sulfated macromolecules as a method for the formation of amyloid plaques. The amyloid protein may be the beta-amyloid protein or the prion protein or the like. Amyoid plaque formation in one embodiment proceeds in vitro and desirably produces amyloid plaques that stain with Congo red and demonstrate a maltese-cross pattern when viewed under polarized light. The method also produces amyloid plaques that demonstrate an "amyloid star" appearance when viewed by transmission electron microscopy. An in vivo assay is also presented for selecting a candidate therapeutic agent for inhibiting or disrupting amyloid plaque deposition or persistence.

机译：将淀粉样蛋白与硫酸化大分子共孵育作为形成淀粉样斑块的方法。淀粉样蛋白可以是β-淀粉样蛋白或the病毒蛋白等。在一个实施方案中，淀粉样蛋白斑的形成在体外进行，并且理想地产生淀粉样蛋白斑，该淀粉样斑被刚果红染色并且当在偏振光下观察时表现出麦芽十字图案。该方法还产生淀粉样蛋白斑，当通过透射电子显微镜观察时，该淀粉样斑显示出“淀粉样星”外观。还提出了用于选择候选治疗剂以抑制或破坏淀粉样斑块沉积或持久性的体内测定法。

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公开/公告号EP1064013A4

专利类型
公开/公告日2005-05-11

原文格式PDF
申请/专利权人 UNIVERSITY OF WASHINGTON;
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申请/专利号EP19990912468
发明设计人 CASTILLO GERARDO;SNOW ALAN D.;
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申请日1999-03-12
分类号A61K38/16;A61K47/20;C07K1/10;C07K17/02;C07K19/00;C07K14/47;A01K67/00;A61K49/00;
国家 EP
入库时间 2022-08-21 22:10:32

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2. Effects of natural-cerebrolysin-containing serum on neurotoxicity and synaptogenesis in amyloid-beta 1-40-induced Alzheimer's disease in vitro models [J] . Yinghong Li, Zhengzhi Wu, Andrew C. J. HuangO, 中国神经再生研究（英文版） . 2009,第002期

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6. Chronic treatment with a novel γ-secretase modulator JNJ-40418677 inhibits amyloid plaque formation in a mouse model of Alzheimers disease [O] . B Van Broeck, J-M Chen, G Tréton, 2011

7. 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