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development of systems of three-dimensional culture in

机译：三维文化体系的发展

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摘要

A process for encapsulating and immobilising mammalian stem cells, ovarian follicular cells, gametes, ovarian follicles or mammalian embryos which are able to auto-organise into three-dimensional structures in vitro, and express biological functions in a manner similar to that which is observed in the organism in vivo is described. The capsules are constituted by: a nucleus containing stem cells, ovarian follicular cells, gametes ovarian follicles or mammalian embryos and/or a biocompatible and/or biodegradable polymer; a semi-permeable membrane constituted by a divalent or trivalent metal ion salt of alginic acid, optionally cross-linked on the inner and/or outer surface and/or on both surfaces, optionally vehicularising a second or more cellular species. The cells and follicles prepared and cultivated using this methodology are used for the in vitro and/or in vivo production of peptides, proteins, antibodies, hormones and hormone precursors, metabolites and catabolites typical of these cellular structures, plasma membranes, nuclear membranes, cytoplasmic organelles, somatic cell nuclei or gametes and embryos.

机译：一种封装和固定哺乳动物干细胞，卵巢滤泡细胞，配子，卵巢滤泡或哺乳动物胚胎的方法，该方法能够在体外自动组织成三维结构，并以类似于在大肠杆菌中观察到的方式表达生物学功能描述了体内生物。所述胶囊由以下物质构成：含有干细胞，卵巢滤泡细胞，配子卵巢滤泡或哺乳动物胚胎和/或生物相容和/或可生物降解的聚合物的细胞核;一种由藻酸的二价或三价金属离子盐构成的半透膜，其任选在内表面和/或外表面和/或两个表面上交联，任选地使第二种或多种细胞种类载体化。使用该方法制备和培养的细胞和卵泡可用于体外和/或体内生产这些细胞结构，质膜，核膜，细胞质典型的肽，蛋白质，抗体，激素和激素前体，代谢产物和分解代谢产物。细胞器，体细胞核或配子和胚胎。

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公开/公告号ITMI20032115A1

专利类型
公开/公告日2005-05-04

原文格式PDF
申请/专利权人 UNIVERSITA DEGLI DUSTDI DI PAVIA;UNIVERSITA DEGLI STUDI DI BOLOGNA;UNIVERSITA DEGLI STUDI DI MILANO;
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申请/专利号IT2003MI02115
发明设计人 RUSSO VINCENZO;STACCHEZZINI SIMONA;FAUSTINI MASSIMO;SPINACI MARCELLA;CONTE UBALDO;ACCORSI PIER ATTILIO;GALEATI GIOVANNA;TORRE MARIA LUISA;
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申请日2003-11-03
分类号
国家 IT
入库时间 2022-08-21 22:15:42

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