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Water-soluble extract from plants of solanum genus, particularly S. incanum and S. nigrum, the preparation process thereof and pharmaceutical compositions for inhibiting cancer/tumour cell growth
Water-soluble extract from plants of solanum genus, particularly S. incanum and S. nigrum, the preparation process thereof and pharmaceutical compositions for inhibiting cancer/tumour cell growth
Described is a water soluble extract from plant of the Solanum genus, which consists of at least 60-90% of solamargine and solasonine and a process for preparing the water soluble plant extract comprising the steps of: a) subjecting plant material from a plant of the Solanum genus to an extraction treatment using an acidic aqueous solution with a pH of 3-5, such that an aqueous solution is obtained; b) adjusting the pH of the solution from step a) to pH 8-10 with a base, such that a precipitate is formed; c) washing the precipitate from step b) with water, followed by drying, such that a dried product is obtained; d) admixing the dried product from step c) with chloroform, followed by addition of a suitable amount of a 100% alcohol, such that a chloroform-alcohol mixture is formed; e) mixing the chloroform-alcohol mixture from step d) with a water/alcohol solution having a predetermined water/alcohol ratio, such that a mixture containing a chloroform-based layer and a non chloroform-based layer is obtained, and; f) removing the chloroform-based layer from the mixture obtained from step e) followed by the addition of a suitable amount of water, and; g) obtaining a supernatant from the resultant mixture of step f) followed by drying the supernatant, such that the resultant dried product is able to be directly dissolved in water to form a yellowish clear transparent aqueous solution. The figureshows a HPLC spectrum of the water-soluble extract from the fruit of Solanum incanum L.. The first peak corresponds to solasonine and the second peak corresponds to solamargine, and the content of solamargine is greater than that of solasonine.
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