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A PROCESS FOR ISOLATION AND PURIFICATION OF bRUGIA MALAYI MICROFILARIAL EXCRETORY - SECRETORY (MF ES-12) AND SOLUBLE (MF S-33 AND MF S-66) GLYCOPROTEINS FOR DIAGNOSIS AND MONITORING OF ACTIVE FILARIAL INFECTION AND FOR MORBIDITY CONTROL IN CLINICAL FILARIASIS
A PROCESS FOR ISOLATION AND PURIFICATION OF bRUGIA MALAYI MICROFILARIAL EXCRETORY - SECRETORY (MF ES-12) AND SOLUBLE (MF S-33 AND MF S-66) GLYCOPROTEINS FOR DIAGNOSIS AND MONITORING OF ACTIVE FILARIAL INFECTION AND FOR MORBIDITY CONTROL IN CLINICAL FILARIASIS
This invention relates to a process for the preparation of brugia malayi microfilarial excretory - secretory (mf ES-22) glycoprotein, which is used for diagnosis and monitoring of active filarial infection. According to the process brugia malayia microfilariae is collected from peritoneal cavities of jirds. Brugia malayi microfilarial excretory-secretory (Bm mf ES) antigen if prepared and then the same is subjected to the step of fractionation by fast protein liquid chromatography (FPLC) so as to obtain protein.
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