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METHOD OF EXPRESSING AND SECRETING SOLUBLE EXTRACELLULAR DOMAINS OF HUMAN GONADOTROPIN HORMONE RECEPTORS AND RECOMBINANT BACULOVIRUS VECTOR FOR USE THERE IN
METHOD OF EXPRESSING AND SECRETING SOLUBLE EXTRACELLULAR DOMAINS OF HUMAN GONADOTROPIN HORMONE RECEPTORS AND RECOMBINANT BACULOVIRUS VECTOR FOR USE THERE IN
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机译：表达和分泌人类促性腺激素受体激素受体和重组杆状病毒载体的胞外域的方法
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摘要
The extracellular domain, or fragment thereof, of a gonadotropin glycoprotein hormone receptor is expressed and secreted in a soluble and functionally hormone-binding form. A recombinant baculovirus transfer vector is constructed to include a gene segment encoding the extracellular domain, or fragment thereof, of the glycoprotein hormone receptor joined in frame with a gene segment encoding a baculovirus signal peptide and operably linked to a baculovirus promoter. Recombinant baculovirus generated by transfection or co-transfection of insect cells are then used to infect insect host cells for the expression and secretion of soluble receptor.
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