首页> 外国专利> Method for selecting transformed plant cells using ethionine and cystathionine gamma synthase as the selection agent and marker gene

Method for selecting transformed plant cells using ethionine and cystathionine gamma synthase as the selection agent and marker gene

机译:以乙硫氨酸和胱硫醚γ合酶为选择剂和标记基因的转化植物细胞的选择方法

摘要

Disclosed are transgenic plants having edible portions that produce methional during processing. The plants contain increased methionine levels such that upon processing of the edible portion(s), methional levels are increased and lead to food products that possess improved flavor stability and/or quality. Plants of the Solanaceous family e.g., potato, tomato and eggplant, and other methional-producing plants including maize and soybean, are preferred plants. Several ways of genetically engineering plants to produce increased free Met levels are disclosed, with introduction of a non-native nucleic acid encoding cystathionine gamma synthase (CGS) and tissue-specific expression of an anti-sense S-adenosyl-methionine synthetase being preferred. Also disclosed are methods for selecting transformed plant cells using ethionine and CGS as the selection agent and marker gene respectively.
机译:公开了具有在加工过程中产生甲硫氨酸的可食部分的转基因植物。植物中蛋氨酸的含量增加,因此在加工可食用部分时蛋氨酸的含量增加,导致食品的风味稳定性和/或品质得到改善。优选茄科的植物,例如马铃薯,番茄和茄子,以及其他产生甲基化的植物,包括玉米和大豆。公开了几种基因工程改造植物以产生增加的游离Met水平的方法,其中引入了编码胱硫醚γ合成酶(CGS)的非天然核酸,并且优选反义S-腺苷甲硫氨酸合成酶的组织特异性表达。还公开了分别使用乙硫氨酸和CGS作为选择剂和标记基因选择转化的植物细胞的方法。

著录项

  • 公开/公告号US2005039231A1

    专利类型

  • 公开/公告日2005-02-17

    原文格式PDF

  • 申请/专利权人 NILGUN E. TUMER;THOMAS LEUSTEK;

    申请/专利号US20040948975

  • 发明设计人 NILGUN E. TUMER;THOMAS LEUSTEK;

    申请日2004-09-24

  • 分类号A01H1/00;C12N15/82;A01H5/00;

  • 国家 US

  • 入库时间 2022-08-21 22:23:12

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