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Apparatus for carrying out this method and detecting DNA interactions and DNA-binding proteins of a number of sample systems and kits
Apparatus for carrying out this method and detecting DNA interactions and DNA-binding proteins of a number of sample systems and kits
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机译：用于执行此方法并检测许多样品系统和试剂盒的DNA相互作用和DNA结合蛋白的设备
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摘要
One 1, DNA binding proteins usually a plurality of, for example, a method of detecting qualitatively and quantitatively the presence of a transcription factor are provided, at least in the sample. In the method of the present invention, one is the substrate DNA one or more probes that correspond to a single DNA binding protein 1 of interest is immobilized on a surface, binding the complex of DNA binding proteins and the respective probe and contacting the sample under conditions sufficient to effect the formation and thereby. Be purified for DNA-binding proteins with one or more samples, or sample may be a cell / nuclear extracts. The detection is then binding complex on the surface of the substrate resulting, it is related to the presence of DNA binding proteins -DNA interactions of interest in the sample. Moreover, devices and systems for use in practicing the method of the present invention are also provided. Variety of different applications, for example, the method of the present invention have found considering the profile of transcription factors that respond detecting the presence of a transcription factor in the sample, to a predetermined stimulus, the use in the screening of therapeutic agents.
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