首页> 外国专利> HEAT-RESISTANT FIREFLY LUCIFERASE, HEAT-RESISTANT FIREFLY LUCIFERASE GENE, NEW RECOMBINANT DNA AND METHOD FOR PRODUCING HEAT-RESISTANT FIREFLY LUCIFERASE

HEAT-RESISTANT FIREFLY LUCIFERASE, HEAT-RESISTANT FIREFLY LUCIFERASE GENE, NEW RECOMBINANT DNA AND METHOD FOR PRODUCING HEAT-RESISTANT FIREFLY LUCIFERASE

机译：耐热全荧光素酶基因，耐热全荧光素酶基因，新的重组DNA及生产耐热全荧光素酶的方法

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摘要

PROBLEM TO BE SOLVED: To provide a method for producing a heat-resistant firefly luciferase by changing specific amino acid residues in a mild type firefly luciferase into hydrophobic amino acid residues.;SOLUTION: This heat-resistant firefly luciferase is characterized by changing the amino acids at the 217- and 315-positions in the amino acid sequence of the mild type firefly luciferase or the amino acids at the positions equivalent to the 217- and 315-positions in the amino acid sequence of Luciola cruciata or Luciola lateralis luciferase into hydrophobic acid residues. The heat-resistant firefly luciferase gene. A vector integrated with the gene. The method for producing the heat-resistant firefly luciferase comprises using the vector to produce the heat-resistant firefly luciferase. The heat-resistant firefly luciferase can efficiently be obtained by the method, and is highly useful on industry.;COPYRIGHT: (C)2005,JPO&NCIPI

机译：解决的问题：提供一种通过将温和型萤火虫荧光素酶中的特定氨基酸残基改变为疏水性氨基酸残基来生产耐热萤火虫荧光素酶的方法;解决方案：该耐热萤火虫荧光素酶的特征在于改变氨基酸萤火虫萤光素萤光素酶的氨基酸序列中217和315位的氨基酸或与十字花Luciola cruciata或侧生Luciolalateralis萤光素酶的氨基酸序列中与217和315位相同的氨基酸酸残基。耐热萤火虫荧光素酶基因。与基因整合的载体。产生耐热萤火虫荧光素酶的方法包括使用载体产生耐热萤火虫荧光素酶。该方法可有效获得耐热萤火虫荧光素酶，在工业上具有很高的应用价值。;版权所有：（C）2005，日本专利商标局

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公开/公告号JP2005245457A

专利类型
公开/公告日2005-09-15

原文格式PDF
申请/专利权人 KIKKOMAN CORP;
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申请/专利号JP20050111777
发明设计人 KIKUCHI MAMORU;FUKUDA MASARU;TATSUMI HIROKI;
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申请日2005-04-08
分类号C12N15/09;C12N9/02;
国家 JP
入库时间 2022-08-21 22:37:29

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